Menadione (vitamin K-3,2-methyl-1,4-naphthoquinone), a redox cycling reagent, generates reactive oxygen intermediates and causes oxidative injury. The addition of menadione to Hep G2 cells produced a timeand concentration-dependent loss of cell viability. Preincubation of Hep G2 cells with low, nontoxic concentrations of menadione increased the viability of the cells against toxic doses of mena...

LPS could induce nuclear translocation of NF-kB in E1Atransfected cells alone.7 In E1A-transformed airway cells, the NF-kB transcription factor is involved in the IL-8 gene and ICAM-1 gene expression after LPS stimulation. In addition, Metcalf8 reported that adenovirus E1A 13S gene product upregulates TNF-a gene. On the other hand, cigarette smokers have increased numbers of neutrophils present...

LPS could induce nuclear translocation of NF-kB in E1Atransfected cells alone.7 In E1A-transformed airway cells, the NF-kB transcription factor is involved in the IL-8 gene and ICAM-1 gene expression after LPS stimulation. In addition, Metcalf8 reported that adenovirus E1A 13S gene product upregulates TNF-a gene. On the other hand, cigarette smokers have increased numbers of neutrophils present...

Activation of the transcription factor NF-kB is critical for the tumor necrosis factor-a (TNF-a)-induced inflammatory response. Here we report the complete gene expression profile from activated microvascular endothelial cells emphasizing the direct contribution of the NF-kB pathway. Human microvascular endothelial cell line-1 (HMEC-1) cells were modified to express dominant interfering mutants...

Gastrointestinal neoplasia seems to be a common consequence of chronic inflammation in the gastrointestinal epithelium. Nuclear factor-kappaB (NF-kB) is an important transcription factor for carcinogenesis in chronic inflammatory diseases and plays a key role in promoting inflammation-associated carcinoma in the gastrointestinal tract. Activation of NF-kB is regulated by several posttranslation...

LPS could induce nuclear translocation of NF-kB in E1Atransfected cells alone.7 In E1A-transformed airway cells, the NF-kB transcription factor is involved in the IL-8 gene and ICAM-1 gene expression after LPS stimulation. In addition, Metcalf8 reported that adenovirus E1A 13S gene product upregulates TNF-a gene. On the other hand, cigarette smokers have increased numbers of neutrophils present...

Single binding sites for transcription factors NF-IL6 and NF-iB are present in the promoter of the interleukin (IL) 6 gene. Previous studies of internally deleted promoter mutants demonstrated that these two sites are important for the transcriptional regulation of this gene. In this report, we describe the synergistic activation of the IL-6 promoter by transcription factors NF-IL6 and NF-KB. C...

Activation of the transcription factor nuclear factor-KB (NF-KB) has been implicated in pancreatic tumorigenesis. We evaluated the effect of a novel NF-KB inhibitor, parthenolide, a sesquiterpene lactone isolated from the herb feverfew, in three human pancreatic tumor cell lines (BxPC-3, PANC-1, and MIA PaCa-2). Parthenolide inhibited pancreatic cancer cell growth in a dose-dependent manner wit...

During human cytomegalovirus (HCMV) infection, a series of regulated events take place following virus binding and entry into the cell, including the upregulation of cellular transcription factors, such as NF-kB, which play an essential role in the viral life cycle. We show here that NF-kB message is induced during HCMV infection and that the induction is biphasic, suggesting an initial inducti...

Li, Li, Jaladanki N. Rao, Barbara L. Bass, and JianYing Wang. NF-kB activation and susceptibility to apoptosis after polyamine depletion in intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol 280: G992–G1004, 2001.—The maintenance of intestinal mucosal integrity depends on a balance between cell renewal and cell death, including apoptosis. The natural polyamines, putrescine, sp...