In Neurospora crassa, the interactions between products of the frequency (frq), frequency-interacting RNA helicase (frh), white collar-1 (wc-1), and white collar-2 (wc-2) genes establish a molecular circadian clockwork, called the FRQ-WC-Oscillator (FWO), which is required for the generation of molecular and overt circadian rhythmicity. In strains carrying nonfunctional frq alleles, circadian r...

The poky (mi-1) strain of Neurospora crassa is a and the mass ratio of large to small subunit RNA useful test organism in which to evaluate methods is -10 :1 (1) . Nonetheless, mitochondrial protein for isolating mitochondrial ribosomes actively synthesis can be observed in poky (2) and cytoengaged in protein synthesis . In poky mitochondria chrome oxidase which is in part synthesized by there ...

In this paper we report on the reactions and enzyme components of transsulfuration in Neurospora crassa, Saccharomyces cerevisiae, Escherichia co& and Salmonella typhimurium, and show that the process is reversible in the higher fungi, and irreversible, in the direction opposite to that of mammalian tissues, in the bacteria. Recently, we have reported the isolation of two cystathionine cleavage...

The activation of transcription by light in the fungus Neurospora crassa requires the White Collar Complex (WCC), a photoreceptor and transcription factor complex. After light reception two WCCs interact and bind the promoters of light-regulated genes to activate transcription. This process is regulated by VVD, a small photoreceptor that disrupts the interaction between WCCs and leads to a redu...

Regulatory circuits have been defined in Neurospora c m s a within which multiple unlinked genes are controlled in a positive fashion by major regulatory genes. It has been suggested that repetitive DNA sequences situated adjacent to structural genes may serve as regulatory sites. Experiments are presented which examine the amount and organization of the repetitive sequences of Neurospora. The...

Radioactive amino acids were incorporated in vivo into Neurospora crassa cells, and the mitochondrial ribosomes were isolated. The incorporat,ion of radioactivity into the proteins of these ribosomes was inhibited by cycloheximide, but not by chloramphenicol. It is therefore concluded that these proteins are synthesized on the cycloheximide sensitive and chloramphenicol insensitive cytoplasmic ...

The Botrytis cinerea homolog (Bc-hch) of Nc-het-c and Pa-hch (vegetative incompatibility loci of Neurospora crassa and Podospora anserina respectively) was cloned and sequenced. The gene structure of Bc-hch is very close to those of Nc-het-c and Pa-hch. A PCR-RFLP approach on a 1171 bp fragment was used to screen polymorphism at this locus among 117 natural isolates of B. cinerea. Restriction p...

Mutants of Neurospora crassa unable to participate in vegetative hyphal fusion (anastomosis) were isolated and characterized. From this analysis, three genes, rcm-1, rco-1 and ham-5, were identified and shown to be required for hyphal fusion. The rcm-1 and rco-1 genes are homologues of the Saccharomyces cerevisiae SSN6 and TUP1 genes, which encode a dimeric transcription factor in yeast. We dem...

Studies on heterokaryons forced between sorbose-resistant (transport-defective) mutans of Neurospora crassa and a sorbose-sensitive (transport-intact) wildtype strain show that mutants sorr A-l and sorr B-57, mapping in separate linkage groups, are recessive to wildtype; a third mutant, not linked to the others, shows intermediate expression of the resistance character for one of two criteria c...

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