Avian influenza virus stocks (A/chicken/Iran/259/1998/(H9N2)) were propagated in the allantoic cavities of 10-day-old embryonated chicken eggs. The harvested suspension was concentrated by polyethylene glycol 6000. Concentrated samples were layered onto sucrose gradient (30%-60%). Both hemagglutinin and neuraminidase were solubilized from purified viruses with Triton X-100 across 30% sucrose gr...

Strain A mouse ascites tumor cells that were treated with neuraminidase (EC 3.2.1.18) (these cells show decreased tumor-forming ability in allogeneic C3H mice) rapidly regenerate sialoglycoproteins at the cell surface during culture. The incorporation of labeled D-glucosamine into membrane glycoproteins of cells that were treated or untreated with neuraminidase proceeds at similar rates. Surfac...

Photoreceptors can segregate inner segment proteins from outer segment proteins. It is uncertain whether such a sorting process occurs during disc morphogenesis in rods, thereby resulting in an outer segment plasma membrane which differs compositionally from the discs. In this study, the plasma membranes of intact, purified frog rod outer segments (ROS) have been labeled with a peanut lectin (P...

The relation of neuraminidase to morbidity and mortality was examined in patients with Haemophilus influenzae, meningococcal, and pneumococcal meningitis. Ten strains of H. influenzae and eight strains of meningococci from infected cerebrospinal fluid (CSF) did not elaborate neuraminidase. Each of 27 strains of pneumococci from infected CSF elaborated both neuraminidase and N-acetylneuraminic a...

It has been known that the agglutinability of human red cells is changed or enhanced by treatments with proteolytic enzymes or neuraminidase. In this paper, the serological properties of agglutinogens developed by proteolytic enzymes (bromelin, ficin, papain, trypsin and pronase) and neuraminidase are investigated by using antisera to trypsin- and neuraminidase-treated red cells. The adsorption...

Received January 14, 2014 It is critical to seek potential alternative treatments for H1N1 infections by inhibiting neuraminidase-1 enzyme. One of the viable options for inhibiting the activity of neuraminidase-1 is peptide drug design. In order to increase peptide stability, cyclization is necessary to prevent its digestion by protease enzyme. Cyclization of peptide ligands by formation of dis...

BACKGROUND The 2009 influenza A(H1N1) pandemic called attention to the limited influenza treatment options available, especially in individuals at high risk of severe disease. Neuraminidase inhibitor-resistant seasonal H1N1 viruses have demonstrated the ability to transmit well despite early data indicating that resistance reduces viral fitness. 2009 H1N1 pandemic viruses have sporadically appe...

Photoreceptors can segregate inner segment proteins from outer segment proteins. It is uncertain whether such a sorting process occurs during disc morphogenesis in rods, thereby resulting in an outer segment plasma membrane which differs compositionally from the discs. In this study, the plasma membranes of intact, purified frog rod outer segments (ROS) have been labeled with a peanut lectin (P...

The Y155H amino acid substitution in the neuraminidase gene (NA) has previously been associated with highly reduced inhibition by neuraminidase inhibitors in the seasonal H1N1 influenza A virus which circulated in humans before the 2009 pandemic. During the 2012/13 epidemic season in Spain, two A(H1N1) pdm09 viruses bearing the specific Y155H substitution in the NA were detected and isolated fr...