The new enzyme-assisted assay for DNA/RNA detection provides real-time fluorescent signal readout along with low limit of detection and high discrimination power toward a single-base substitution. Requiring only two new unmodified DNA oligonucleotides for the detection of each new analyte, the assay is an efficient tool for low-cost analysis of multiple analytes.

Multivariate calibration (MVC) and near-infrared (NIR) spectroscopy have demonstrated potential for rapid analysis of melamine in various dairy products. However, the practical application of ordinary MVC can be largely restricted because the prediction of a new sample from an uncalibrated batch would be subject to a significant bias due to matrix effect. In this study, the feasibility of using...

In this paper, we report on the preparation of novel cross-reactive optical microsensors for high-speed detection of low-level explosives and explosives-like vapors. Porous silica microspheres with an incorporated environmentally sensitive fluorescent dye are employed in high-density sensor arrays to monitor fluorescence changes during nitroaromatic compound (NAC) vapor exposure. The porous sil...

Molecular Beacon (MB) probes have been extensively used for nucleic acid analysis because of their ability to produce fluorescent signal in solution instantly after hybridization. The indirect binding of MB probe to a target analyte offers several advantages, including: improved genotyping accuracy and the possibility to analyse folded nucleic acids. Here we report on a new design for MB-based ...

Technological advancements have increased the analytical sensitivity of a wide range of assays, permitting the analysis of vanishingly small concentrations of analyte. Accordingly, the minimum quantity of sample needed for analysis has become increasingly driven by the ever greater analytical sensitivity of laboratory methods with little consideration of the influence of decreasing sample mass ...

Carrier signal detection is a complicated and essential task in many domains because it demands quick response to the existence of several carriers wideband, while also precisely predicting each carrier signal’s frequency centers bandwidths, including single-carrier multi-carrier modulation signals. Multi-carrier signals, such as FSK OFDM, could be incorrectly recognized signals by using spectr...

Two Divisions of the International Union of Pure and Applied Chemistry (IUPAC), namely Physical Chemistry (Commission I.7 on Biophysical Chemistry, formerly Steering Committee on Biophysical Chemistry) and Analytical Chemistry (Commission V.5 on Electroanalytical Chemistry), have prepared recommendations on the de®nition, classi®cation and nomenclature related to electrochemical biosensors; the...

Traditional antibody-based affinity sensing strategies employ multiple reagents and washing steps and are unsuitable for real-time detection of analyte binding. Aptamers, on the other hand, may be designed to monitor binding events directly, in real-time, without the need for secondary labels. The goal of the present study was to design an aptamer beacon for fluorescence resonance energy transf...

We propose a novel quantum nonlinear interferometer design that incorporates passive PT symmetric coupler sandwiched between two sections where signal-idler photon pairs are generated. The PT-symmetry enables efficient coupling of the longer-wavelength idler photons and facilitates sensing losses in second waveguide exposed to analyte under investigation, whose absorption can be inferred by mea...