نتایج جستجو برای: native bacteria
تعداد نتایج: 279521 فیلتر نتایج به سال:
FtsH is a membrane-bound protease that plays crucial role in proteolytic regulation of many cellular functions. It universally conserved bacteria and responsible for the degradation misfolded or misassembled proteins. A recent study has determined structure bacterial detergent micelles. To properly function native-like environment, we reconstituted complex into lipid nanodiscs. We found membran...
We systematically studied the origin of surface patterns observed on single Sinorhizobium meliloti bacterial cells by comparing the complementary techniques atomic force microscopy (AFM) and scanning electron microscopy (SEM). Conditions ranged from living bacteria in liquid to fixed bacteria in high vacuum. Stepwise, we applied different sample modifications (fixation, drying, metal coating, e...
UDP-MurNAc-pentapeptide derivative bacterial cell-wall precursors were synthesized as effective tools for surface display on living bacteria. Lactobacilli were incubated in the ketone-modified precursor-containing medium, and the ketone moiety was displayed on the bacterial surface through cell-wall biosynthesis. Oligomannose was coupled with the ketone moiety on the bacterial surface via a ami...
Copper kills bacteria rapidly by a mechanism that is not yet fully resolved. The antibacterial property of copper has raised interest in its use in hospitals, in place of plastic or stainless steel. On the latter surfaces, bacteria can survive for days or even weeks. Copper surfaces could thus provide a powerful accessory measure to curb nosocomial infections. We here investigated the effect of...
Bacterial community compositions (BCC) associated with the cladoceran Bosmina coregoni and the cyclopoid copepod Thermocyclops oithonoides in oligotrophic Lake Stechlin versus eutrophic Lake Dagow (northeastern Germany) were compared using molecular techniques. We also transplanted the zooplankton from their native lake to the other lake, and studied changes in their associated BCC as a result ...
The native l-serine deaminase (l-serine hydrolyase, deaminating, EC 4.2.1.13) of Escherichia coli K-12, which seems to be a very labile protein, is rather stable in concentrated solution. Dilution rapidly inactivates it, but in the presence of a saturating concentration of l-serine the molecule is protected from inactivation. It is a very specific enzyme; l-serine is the sole substrate with a K...
Polyhydroxybutyrate (PHB) is a carbon reserve found in some bacteria, and under nutrient limiting conditions accumulates intracellularly in the form of inclusion bodies. These inclusions contain proteins, and the PHI3 within the inclusions exists in an amorphous state. In this study a procedure to recover native PHB inclusions was developed, and the isolated inclusions were characterized using ...
In the late 19th and early 20th centuries, two medicine circles existed in Indian country: one Native and one Euro-American. Traditional doctors among First Nations peoples approached disease in spiritual ways and also used herbal medicine to treat their patients. First Nations people tried to treat infectious diseases brought by newcomers through plant medicine, ritual, and ceremony. Generally...
Insertion of folded proteins into the outer membrane of Gram-negative bacteria is mediated by the essential β-barrel assembly machine (Bam). Here, we report the native structure and mechanism of a core component of this complex, BamE, and show that it is exclusively monomeric in its native environment of the periplasm, but is able to adopt a distinct dimeric conformation in the cytoplasm. BamE ...
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