A simple, precise, rapid and accurate RP-HPLC method has been developed for the simultaneous estimation of Amlodipine besylate and Hydrochlorthiazide in tablet formulations. The chromatographic separation was achieved on a Shimadzu Symmetry C18 column (250 mm x 4.6mm, 5.0 μ particle size) using Methanol: Acetonitrile: 50mM Na2HPO4 pH7.0 (60:20:20v/v/v) with 1% triethylamine. Flow rate was 1ml/m...

New UPLC method was developed for determination of sitagliptin and metformin using Symmetry C18 column (100 mm × 2.1 mm, 2.2 μm) and isocratic elution (methanol 20%), pH (3.5) as a mobile phase. The ultraviolet detector was operated at 220 nm and the column temperature was 50°C. Linearity parameters were acceptable over the concentration ranges of 2-12 μgml(-1) and 5-35 μgml(-1) for sitagliptin...

To establish the method for qualitative and quantitative determination of gallic acid in Herba Gei. Gallic acid content is determined by HPLC with Diamonsil C18 column as the stationary phase, under the conditions of methanol-0.1% phosphoric acid in water (10:90) mobile phase, 271 nm detection wavelength, and 40°C column temperature. Peak area of gallic acid shows a good linearity with the inje...

A rapid separation of the ten nuclearly-encoded subunits of mitochondrial cytochrome c oxidase, and ten out of the eleven subunits of cytochrome bc1, was achieved using a short, 50 mm C18-reversed-phase column. The short column decreased the elution time 4-7 fold while maintaining the same resolution quality. Elution was similar to a previously published protocol, i.e., a water/acetonitrile elu...

A new simple isocratic RP-HPLC method was developed for the determination of milnacipran in bulk drug and in its capsules formulations. The mobile phase selected was methanol: acetonitrile:0.1% O-phosphoric acid in the ratio of 55:35:10%(v/v) and Zodiac C18 column with dimensions (250X4.6mm, 5μ)was used as stationary phase column temperature is ambient throughout the method. The flow rate and w...

Background: Liver abscesses, refractory to medical therapy requires percutaneous drainage by catheter placed under ultrasonography guidance is considered as the treatment of choice. Pigtail catheter is most commonly used for this purpose which gets repeatedly blocked, requires regular fl ushing and takes a long time to evacuate the abscess. Materials and Methods: In this study, we compared the ...

Tocopherols extracted from plasma with methanol or from platelets with chloroform/methanol were injected in methanol on a reversed-phase (C18) "high-performance" liquid-chromatographic column and eluted with water/methanol (2/98, by vol) at a flow rate of 1.4 mL/min. A "high-performance" spectrophotofluorometer was used for detection. Analytical recoveries ranged from 89 to 106%. The response w...

The suitability of analytical protocol for determination of sulfonamides in honey was investigated. Sulfonamides were extracted from honey with acetate buffer, and cleaned up by solid phase extraction (SPE) procedure. The liquid chromatography (LC) separation was carried out on RP C18 column and sulfonamides were monitored with fluorescence detector, after pre-column derivatisation. Recoveries ...

We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified molecule fr...

Application of C18 monolithic silica capillary columns in HPLC coupled to ion trap mass spectrometry detection was studied for probing the metabolome of the model plant Arabidopsis thaliana. It could be shown that the use of a long capillary column is an easy and effective approach to reduce ionization suppression by enhanced chromatographic resolution. Several hundred peaks could be detected u...