Glucose-l-phosphoric acid was first isolated from minced and washed frog muscle which had been incubated for 3 hour in phosphate buffer containing small amounts of adenylic acid (2). Under these conditions part of the glucose-l-phosphoric acid (l-ester) was converted to hexose-6-phosphoric acid (8ester), so that fractional crystallization of the brucine salts had to be resorted to as a means of...

This report describes the separation of components from a 4-coumarate:CoA ligase assay by means of liquid chromatography. With the aid of polyamide column chromatography it is possible to enrich and isolate chromatographically and UV spectroscopically pure p-coumaroyl-CoA using as a solvent 0.01% NH4OH in methanol subsequent to water and methanol alone. High performance liquid chromatography on...

Two new, rapid, precise, accurate and specific chromatographic methods were described for the simultaneous determination of olmesartan medoxomil and hydrochlorothiazide in combined tablet dosage forms. The first method was based on reversed phase liquid chromatography using an Eurosphere 100 RP C18 column (250 x 4.6 mm ID, 5 mum). The mobile phase was methanol-0.05% o-phosphoric acid (60:40 v/v...

Structural preservation of complex biological systems from the subcellular to whole organism level in robust forms, enabling dissection and imaging while preserving 3D context, represents an enduring grand challenge in biology. Here we report a simple immersion method for structurally preserving intact organisms via conformal stabilization within silica. This self-limiting process, which we ref...

The current essay analyzes the anti-corrosion properties of epoxy primers including anti-corrosion pigment calcium hydrogen orthophosphate and hydrophobic nano silica with various percentages optimized by Taguchi experimental design. The impact of influential factors such as the percentage of anti-corrosion pigments, percentage of nano silica and pigment to binder ratio on anti-corrosion proper...

Nanofibrous membranes, consisting of a poly(L-lactic acid) (PLLA)-silica xerogel hybrid material, were successfully fabricated from a hybrid sol using the electrospinning technique for guided bone regeneration (GBR) application. These hybrid nanofibers exhibited a homogeneous and continuous morphology, with a nano-sized dispersed silica xerogel phase in the PLLA fiber matrix. The mechanical pro...

A new concept of designing and synthesizing highly dispersed ionic liquids was developed through physical confinement or encapsulation of them into silica gel matrix with sol-gel process. A series of silica gel confined ionic liquids were synthesized through this process and characterized by diffuse-reflectance infrared Fourier transform spectroscopy (DRIFTS) and FTRaman analysis, and abnormal ...

Selected alkaloids are chromatographed on cyanopropyl-silica thin layers using various nonaqueous and aqueous eluents. Because of the strong retention of these basic compounds, nonaqueous eluents containing medium polar diluents, strongly polar modifiers, and silanol blockers (ammonia or diethylamine) are required for separation. Likewise, aqueous eluents containing modifiers (acetonitrile, met...

The effect of different parameters including: nano silica content, curing temperature, type of hardener, and flash-off time on mechanical and optical properties of polyurethane PU based clearcoat was investigated via standard Taguchi L9 method. Dispersion of nano silica in the resultant nanocomposites was explored by scanning electron microscopy SEM. SEM images showed a fine dispersion through ...