Invasive aspergillosis (IA) is one of the most common life-threatening complications in immunocompromised patients. Voriconazole is currently the drug of choice for IA treatment. However, some patients with IA suffer clinical deterioration despite voriconazole therapy. Management of voriconazole-refractory IA remains challenging; no useful recommendations have yet been made. Voriconazole-refrac...

Liposomes modified with TAT peptide (TATp-liposomes) showed fast and efficient translocation into the cell cytoplasm with subsequent migration into the perinuclear zone. TATp-liposomes containing a small quantity (<or=10 mol %) of a cationic lipid formed firm noncovalent complexes with DNA. Here, we present results demonstrating both in vitro and in vivo transfection with TATp-liposome-DNA comp...

OBJECTIVE This study aims to report a case of accelerated metabolism of voriconazole in a patient with cerebral aspergillosis. CASE SUMMARY A 36-year-old woman developed cerebral aspergillosis after immunosuppressive treatment for suspected atypical hemolytic uremic syndrome/thrombotic thrombocytopenic purpura. She was treated with voriconazole using therapeutic drug monitoring to guide dosin...

We describe two patients with fungal keratitis refractory to standard antifungal therapy whose conditions were managed with voriconazole. The first case is a patient with endophthalmitis and corneal ulcer due to Candida parapsilosis after receiving a corneal transplant. The patient was treated with amphotericin but showed no signs of improvement. Topical voriconazole, oral voriconazole, and int...

TAKADA, G., ONODERA, II. and TADA, K. Tissue Distribution of Unentrapped or Liposome-Entrapped 1311-Labeled f3-Galactosidase Injected into Rats. Tohoku J. exp. Med., 1981, 134 (2), 103-114 Either unentrapped (free) or liposomeentrapped 131I-labeled 9-galactosidase was injected into rats from tail veins. Tissue distribution and intracellular localization of the radioactivity of both sources were...

Background Voriconazole therapy for fungal infections usually continues several years and is often administered on an outpatient basis. Maintaining the voriconazole plasma concentration in therapeutic range highly important effective therapy; however, it difficult to obtain sufficient information assess outpatients. Therefore, we developed a method simultaneously measure concentrations of its m...

Gentamicin was successfully incorporated into neutral, anionic, and cationic liposomes, and the percentage of gentamicin incorporated was found to be a function of lipid concentration. Gentamicin did not leak out of the liposomes over a 3-week period at 4 degrees C. When liposome-associated gentamicin was administered intravenously to rabbits, its serum half-life was greatly prolonged. Intragas...

BACKGROUND Voriconazole is the therapy of choice for aspergillosis and a new treatment option for candidiasis. Liver disease, age, genetic polymorphism of the cytochrome CYP2C19, and comedications influence voriconazole metabolism. Large variations in voriconazole pharmacokinetics may be associated with decreased efficacy or with toxicity. METHODS This study was conducted to assess the utilit...

With the advent of multifunctional nano delivery systems, simultaneous imaging and therapy aspires to detect and treat tumors at a very early stage with promising out‐ comes. In this context, numerous anti-cancer drug/gene delivery systems have been ex‐ plored with the primary aim to increase the treatment efficacy without compromising safety. Secondary goals include enhancing bioavailability, ...

We have utilized plant-based transient expression to produce tobacco mosaic virus (TMV)-based nano-rods of predetermined lengths. This is achieved by expressing RNAs containing the TMV origin of assembly sequence (OAS) and the sequence of the TMV coat protein either on the same RNA molecule or on two separate constructs. We show that the length of the resulting nano-rods is dependent upon the l...