In fat cells isolated from the parametrial adipose tissue of rats, the addition of purified adenosine deaminase increased lipolysis and cyclic adenosine 3’: 5’-monophosphate (cyclic AMP) accumulation. Adenosine deaminase markedly potentiated cyclic AMP accumulation due to norepinephrine. The increase in cyclic AMP due to adenosine deaminase was as rapid as that of theophylline with near maximal...

In adipocyte membranes, cholera toxin may ADP-ribosylate the islet-activating protein (IAP) substrate, under certain conditions. Covalent modification is maximal in the absence of a guanosine triphosphate; in the presence of 5'-guanylylimidodiphosphate, incorporation of [32P]ADP-ribose is markedly reduced. ADP-ribosylation by cholera toxin has similar functional consequences as does IAP-mediate...

Propylene oxide (PO) and acrylonitrile epoxide (ANO) are 3-carbon epoxides that are direct-acting mutagens. PO is a rodent carcinogen, and ANO has been postulated to be the ultimate carcinogenic form of acrylonitrile (AN). We have studied the reactions of these agents with 2'-deoxynucleosides and in vitro with calf thymus DNA at pH 7.0 to 7.5 and 37 degrees C. PO was reacted with DNA for 10 hr ...

Pertussis toxin (PT), a protein produced by Bordetella pertussis, was studied for its effect on lipolysis in isolated rat epididymal adipocytes. Exposure of adipocytes to pertussis toxin resulted in a significant increase in cyclic AMP levels and lipolysis after a lag of 1-2 hr. Both the maximal rate of lipolysis and the time lag (beyond 1 hr) were PT concentration-dependent. Heat treatment (95...

Background—Animal model studies have shown that the colon tumour promoting eVect of dietary fat depends not only on the amount but on its fatty acid composition. With respect to this, the eVect of n9 fatty acids, present in olive oil, on colon carcinogenesis has been scarcely investigated. Aims—To assess the eVect of an n9 fat diet on precancer events, carcinoma development, and changes in muco...

Accuracy in translation of the genetic code into proteins depends upon correct tRNA-mRNA recognition in the context of the ribosome. In human tRNA(Lys,3)UUU three modified bases are present in the anticodon stem-loop--2-methylthio-N6-threonylcarbamoyladenosine at position 37 (ms2t6A37), 5-methoxycarbonylmethyl-2-thiouridine at position 34 (mcm5s2U34) and pseudouridine (psi) at position 39--two ...

Among more than 140 naturally occurring RNA modifications have been identified, N6-methyladenosine (m6A) is the most abundant messenger RNA (mRNA) modification in eukaryotic organisms. A group of demethylases, methyltransferase and m6A-specific binding proteins in mammals, plants as well as yeast are in support of the regulatory functions of this RNA modification. Recent years, with the fast de...

In recent years, the research interest in m6A has remained high, and reason is that N6-methyladenosine (m6A) most common reversible methylation modification of eukaryotic mRNA plays a significant role tumorigenesis.

Abstract The nutritional quality of milk, which is determined by its fatty acid (FA) composition, and the candidate gene stearoyl-CoA desaturase (SCD) can be used in conjunction with these traits to improve dairy products. aim this study was identify single nucleotide polymorphism (SNP) SCD associations milk composition FA profiles Najdi sheep 72 multiparous ewes under same feeding system. Milk...