Chronic lymphocytic leukemia (CLL) is a malignant disorder of B cell origin, with low incidence in Asian populations. In this study we investigated the HLA-class I A and B allele frequencies in 87 Iranian CLL patients and 64 healthy controls using sequence specific primer-polymerase chain reaction (SSP-PCR) technique. Our results showed increased frequencies of HLA-A11:01 (p=0.02) and HLA-B35:0...

The presentation of antigenic peptides by major histocompatibility complex (MHC) class II to CD4+ T cells is crucial to initiate immune responses. We developed a new system for delivery of an antigenic peptide to the MHC class II pathway, using the invariant chain (Ii). We designed a mutated human p33-form Ii, CLIP-substituted Ii, in which streptococcal M12p55-68 (RDLEQAYNELSGEA) was substitute...

The t(14;18)(q32;q21) is a cytogenetic hallmark of follicular lymphoma and also occurs in approximately 20% of diffuse large B-cell lymphomas of follicle center cell origin. Relatively few cases of chronic lymphocytic leukemia/small lymphocytic lymphoma with t(14;18) have been reported previously. We report the clinicopathologic, cytogenetic, and molecular genetic features of 12 patients with c...

Variants of four hybridoma lines secreting antibodies specific for either trinitrophenyl (TNP) or sheep erythrocytes (SRBC) were isolated which have lost either the specific heavy (H) chain or the specific light (L) chain. They were fused to lipopolysaccharide-stimulated mouse spleen cells and the resulting secondary hybridomas were screened for the restoration of the original antibody specific...

An artificial amyloid-based redox hydrogel was designed for mediating electron transfer between a [NiFeSe] hydrogenase and an electrode. Starting from mutated prion-forming domain of fungal protein HET-s, hybrid containing single benzyl methyl viologen moiety synthesized. This able to self-assemble into structurally homogenous nanofibrils. Molecular modeling confirmed that the groups are aligne...

background : immunogenicity of streptokinase (stk), as a thrombolytic drug, have limited its clinical use. elimination of the amino acid residues that are responsible for immunogenicity while don’t affect the bioactivity of stk is worthy. recently, we modified the stk through elimination of 42 amino acids from its’ c-terminal and assessed its bioactivity in vitro . objective : in this study, bi...

Congenital ichthyoses (CI), inherited scaling disorders, have an impaired epidermal barrier, but ceramide (Cer) changes are poorly understood. Using untargeted lipidomics, we compared Cer profiles of tape-stripped skin from 77 patients with 6 subtypes CI and 19 matched controls. In CI, 636 species were found, including 24 low-abundance Cers not in healthy skin. 221 significantly (>2-fold; adj. ...

The adaptive immune system of jawed vertebrates generates a highly diverse repertoire antibodies to meet the antigenic challenges constantly evolving biological ecosystem. Most diversity is generated by two mechanisms: V(D)J gene recombination and somatic hypermutation (SHM). SHM introduces changes in variable domain antibodies, mostly regions that form paratope, yielding with higher antigen bi...

Steroidal glycoalkaloids (SGAs) existing in most potato tissues are toxic to humans when the fresh weight is over 200 mg kg?1, and high SGAs content would also damage quality of tubers. Sterol side chain reductase 2 (StSSR2) a key enzyme biosynthetic potatoes. To reduce concentration tetraploid cultivated (Solanum tuberosum L.), gene StSSR2 was edited by CRISPR/Cas9 system. A total number 64 mu...