نتایج جستجو برای: multiplex ligation dependentprobe amplification

تعداد نتایج: 97340  

Journal: :Molecular genetics and metabolism 2010
Danielle Crompton Pauline K Rehal Lesley MacPherson Katharine Foster Peter Lunt Imelda Hughes Angela F Brady Michael G Pike Susanna De Gressi Neil V Morgan Carol Hardy Matthew Smith Fiona MacDonald Eamonn R Maher Manju A Kurian

Phospholipase associated neurodegeneration (PLAN) comprises a heterogeneous group of autosomal recessive neurological disorders caused by mutations in the PLA2G6 gene. Direct gene sequencing detects approximately 85% mutations in infantile neuroaxonal dystrophy. We report the novel use of multiplex ligation-dependent probe amplification (MLPA) analysis to detect novel PLA2G6 duplications and de...

2017
Jing Wang Xue Yang Haofeng Chen Xuewei Wang Xiangyu Wang Yi Fang Zhenyu Jia Jidong Gao

RNA in formalin-fixed and paraffin-embedded (FFPE) tissues provides large amount of information indicating disease stages, histological tumor types and grades, as well as clinical outcomes. However, Detection of RNA expression levels in formalin-fixed and paraffin-embedded samples is extremely difficult due to poor RNA quality. Here we developed a high-throughput method, Reverse Transcription-M...

2015
Emmanuelle Souzeau Melanie Hayes Jonathan B. Ruddle James E. Elder Sandra E. Staffieri Lisa S. Kearns David A. Mackey Tiger Zhou Bronwyn Ridge Kathryn P. Burdon Andrew Dubowsky Jamie E. Craig

PURPOSE To evaluate the prevalence and the diagnostic utility of testing for CYP1B1 copy number variation (CNV) in primary congenital glaucoma (PCG) cases unexplained by CYP1B1 point mutations in The Australian and New Zealand Registry of Advanced Glaucoma. METHODS In total, 50 PCG cases either heterozygous for disease-causing variants or with no CYP1B1 sequence variants were included in the ...

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