We present the draft genome sequence of methicillin-resistant Staphylococcus aureus strain CBD-635, from the USA100 lineage. This is a sepsis isolate obtained from Tampa General Hospital. This strain is spa type t003 and multilocus sequence typing (MLST) type ST5, and it has been used by our group in the study of novel antimicrobial chemotherapeutics.

Streptococcal disease and columnaris are common in tilapia aquaculture across the globe. Coinfections also but often overlooked or not reported. In present study, we describe clinical presentation of streptococcosis coinfection diseased Nile ( Oreochromis niloticus ), phenotypic (Lancefield grouping, hemolytic pattern, biochemical characteristics, antibiotic susceptibility) genotypic (multilocu...

The accuracy of the Cronobacter biotyping scheme was compared with the 7-loci multilocus sequence typing scheme. Biotyping did not reliably assign species level identification, as only half (17/31) of the biotype variants were unique to any of the seven Cronobacter species and the remaining biotypes were shared across the genus.

Streptomycetes are a complex group of actinomycetes that produce diverse bioactive metabolites of commercial significance. Systematics can provide a useful framework for identifying species that may produce novel metabolites. However, previously proposed approaches to the systematics of Streptomyces have suffered from either poor interlaboratory comparability or insufficient resolution. In part...

We investigated the genetic variability within and between cattle breeds. The polymorphisms of 17 microsatellites were studied in 220 unrelated animals belonging to four Italian beef cattle breeds (Chianina, Marchigiana, Romagnola, and Piemontese). Variations of allelic frequencies were examined to characterize the breeds and their relationships. Wahlund coefficients, Polymorphism Information C...

Mycobacterium xenopi is an opportunistic mycobacterial pathogen of increasing clinical importance. Surveillance of M. xenopi is hampered by the absence of tools for genotyping and molecular epidemiology. In this study, we describe the development and evaluation of an effective multilocus sequence typing strategy for M. xenopi.

There have been many efforts to develop Bacillus anthracis detection assays, but the problem of false-positive results has often been encountered. Therefore, to validate an assay for B. anthracis detection, it is critical to examine its specificity with the most closely related Bacillus isolates that are available. To define the most closely related Bacillus isolates to B. anthracis in our Baci...

The family Dermateaceae belongs to the Helotiales order, class Leotiomycetes, and consists of 14 genera. In this study, we introduce new genus Pseudoxenochalara gen. nov. family, which is supported by morphological observations multilocus phylogenetic analysis. Partial sequences loci encoding ?-tubulin (BenA), ribosomal polymerase II second largest subunit (RPB2), 28S rDNA (LSU) internal transc...