Early diagnosis of cerebrovascular disease requires the accurate identification of brain regions with compromised cerebral perfusion pressure (CPP). Current clinical measures of CPP are invasive and lack regional information. Dynamic contrast-enhanced imaging provides a means of looking at regional cerebral hemodynamics. The purpose of this study was to determine if any of the parameters associ...

In comparison to monolayer cells, MCTS has been claimed as more suitable candidate for studying drug penetration due to the high resemblance to solid tumors. However, the cultivation of MCTS is cumbersome, time consuming, and most technique fail to generate spheroids with uniform sizes. Therefore, the application of spheroid cultures in high throughput screening has been rather limiting. Beside...

Abstract PURPOSE Magnetic resonance spectroscopy (MRS) is a quantitative technique based on the chemical shift of protons that allows metabolic profile tissues to be analyzed and characterized. Standard glial tumor analysis comprises Creatine (Cr), N-acetil aspartato (NAA) Choline (Cho) metabolites, usually altered in brain pathologies (low grade -LGT-, high-grade cancer -HGT-, metastasis -MTT-...

INTRODUCTION Quantitative cerebral perfusion has been achieved via the Bookend technique [1,2] using dynamic susceptibility contrast (DSC) MRI and T1 changes in normal white matter (WM) in relation to the changes in the blood pool in a calibration slice, after contrast injection. Quantitative cerebral blood flow (qCBF), quantitative cerebral blood volume (qCBV) and mean transit time (MTT) measu...

Inconsistencies in the half-maximal (50%) inhibitory concentration (IC50) data for anticancer chemotherapeutic agents have yielded irreproducible experimental results and thus reciprocally contradictory theories in modern cancer research. The MTT assay is currently the most extensively used method for IC50 measurements. Here, we dissected the critical reasons behind MTT-dependent IC50 inconsist...

Background Oleanolic acid (OA) has notable anti-inflammatory and anti-tumor effects, but the role of OA in cerebral ischemia-reperfusion injury (CIRI) not been reported so far. Methods Oxygen glucose deprivation/reoxygenation (OGD/R) model was induced PC12 cells. MTT assay used to detect cell viability cells, while ELISA detected expression TNF-?, IL-1? IL-6. The superoxide dismutase (SOD), mal...

One major method used to evaluate the biocompatibility of porous tissue engineering scaffolding materials is MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The MTT cell viability assay is based on the absorbance of the dissolved MTT formazan crystals formed in living cells, which is proportional to the number of viable cells. Due to the strong dye sorption capability ...

In this paper, we discuss the use of the Meaning-Text Theory (MTT) of [Mel88] in a controlled language (CL) application. We show that MTT defines a linguistic framework which is ideally suited for the definition and automation of CL rules. In the paper, we first briefly present a CL system based on MTT. We then discuss MTT in more detail. We show how CL-specific information can be represented s...

This study aimed to evaluate the biological functions of excision repair cross complementation goup 1 (ERCC1) in cell proliferation, cell cycle, invasion and cisplatin response of non-small cell lung cancer (NSCLC) cells. Firstly, ERCC1 gene was successfully transfected into H1299 cells by gene cloning and transfection techniques. Then, cell proliferation was determined with the cell growth cur...