To investigate the effect of long non coding RNA MAGI2-antisense 3 on proliferation, invasion and migration cervical cancer cells underlying mechanism. SiHa were divided into plasmid cloning DNA group, DNA-MAGI2-antisense anti-microRNA-negative control anti-microRNA-31-5p 3+microRNAnegative group 3+microRNA-31-5p group; quantitative reverse transcription polymerase chain reaction was used to de...

A study of the subcellular localization of the nicotinamide adenine dinucleotide (NADH)-3-(4, 3-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) oxido-reductase systems in Mycobacterium was presented. Evidence based on starch gel electrophoresis and different responses of the subcellular fractions to heat inactivation suggested the existence of more than one enzyme responsible for the...

Abstract Cell encapsulation is an ideal approach for the replacement of pancreatic function in Type 1 diabetes. Poor biocompatibility microcapsules generates inflammatory response implantation site and induces fibrosis infiltration, which causes microencapsulated cell death graft failure. To prevent inflammation after implantation, composite that exhibit anti‐inflammatory properties were design...

The current research was conducted to investigate the chemical profile, antiproliferative, and antioxidant activities of methanol extracts obtained by two different methods including maceration Soxhlet from Berberis hispanica Boiss. & Reut. Antiproliferative were evaluated MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay in four human cancer cell lines prostate (LnC...

Mangrove endophytes have recently gained considerable attention due to their diversity and abundance of novel bioactive secondary metabolites. Despite the fact that Streptomyces species are producers more than 75% commercialized antibiotics, associated with extremely widespread mangrove plant Avicennia marina remain poorly characterized. In this study, nine actinomycetes were isolated from A. g...

To investigate the effect of emodin combined with Cp-1 on proliferation and apoptosis bladder cancer cell line BIU-87. BIU-87 cells were randomly divided into control group, group. Flow cytometry was used to detect cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay Western blot reverse transcription–polymerase chain reaction recepteur d’origine nantais, Caspase-3 B-cell ...

Natural products have been studied to provide alternative treatments against human diseases as they various medicinal properties. One of these natural is substances in the class flavonoids. These bioactive molecules antioxidant, anti-inflammatory and antitumor activities. The number studies focusing on increasing discover new therapeutic agents such cancer. We aimed determine in-vitro anticance...

OBJECTIVES The aim of this study was to assess the cytotoxic effects and osteogenic activity of recombinant human bone morphogenetic protein (rhBMP2) and nano-hydroxyapatite (n-HA) adjacent to MG-63 cell line. MATERIALS AND METHODS To assess cytotoxicity, the 4,5-dimethyl thiazolyl-2,5-diphenyl tetrazolium bromide (MTT) assay was used. Alkaline phosphatase (ALP) activity and osteogenic activi...

This study was conducted to examine the antioxidative and neuroprotective effects of Paeonia lactiflora pall (PLE). Total phenolic content of PLE was 89.65 mg of gallic acid equivalent per gram of PLE. IC(50) values for reducing power, hydrogen peroxide scavenging activity, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were 297.57, 3.33, and 32.74 microg, respectively. Th...

Gallic acid (GA) comes from benzoic or, more specifically, 3,4,5-tryhydroxybenzoic, which derives the phenolic of non-flavonoid part polyphenol compound. It is found ubiquitously in various plants and fruits, such as grapes, gallnuts, pomegranates, tea leaves [1]. Many scientific journals articles reported on pharmacological properties photochemical like GA, has antioxidant properties, antimicr...