In the current issue of Clinical Chemistry, Braun et al. [1] describe a mutational analysis method that combines primer oligo base extension (PROBE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). This application further extends the use of MS for genetic analysis and suggests a possible role in the clinic. However, what is the long-term potentia...

BACKGROUND Preoperative fasting is a requirement to be made by anesthesiologists, but they generally depend on nurses to ensure it is carried out by patients. Lack of updated knowledge among nurses may cause complications. METHOD The objective of this study was to understand the knowledge of nurses about preoperative fasting. The multi-unit study was conducted in the units of our hospitals us...

Epidemiological and forensic analyses of bioterrorism events involving Bacillus anthracis could be improved if both variable number tandem repeats (VNTRs) and single nucleotide polymorphisms (SNPs) could be combined on a single analysis platform. Here we present the use of electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) to characterize 24 allel...

This study aimed to assess the occurrence of toxigenic fungi and mycotoxin contamination in stored wheat grains by using advanced molecular and analytical techniques. A multiplex polymerase chain reaction (PCR) strategy was established for rapid identification of mycotoxigenic fungi, and an improved analytical method was developed for simultaneous multi-mycotoxin determination in wheat grains b...

We present a mass spectrometry (MS)-based nucleoside-specific mass-tagging method to validate genomic DNA sequences containing ambiguities not resolved by gel electrophoresis. Selected types of (13)C/(15)N-labeled dNTPs are used in PCR amplification of target regions followed by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF)-MS analysis. From the mass difference between ...

A rapid approach to the 16S rRNA gene (16S rDNA)-based bacterial identification has been developed that combines uracil-DNA-glycosylase (UDG)-mediated base-specific fragmentation of PCR products with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). 16S rDNA signature sequences were PCR-amplified from both cultured and as-yet-uncultured bacteria in the...

conclusions the results of this study showed a possible association between cmv infection and ms. further experimental and epidemiological studies using case-control approaches are needed to confirm this association. results anti-cmv was positive in 65 (79.3%) and 69 (77.5%) of the ms patients and healthy subjects, respectively (p= 0.853). similarly, 23 (28%) and 2 (2.2%) patients were positive...

BACKGROUND & OBJECTIVES Dental caries is an infectious, transmissible disease. Maternal transfer of mutans streptococci (MS) has been a subject of research. The aim of this study was to evaluate the transmission of MS from mother to children through genetic analysis. METHODS Thirty mother-child pairs were included and divided into three groups according to the age of the children. Saliva samp...

Background: Multiple Sclerosis (MS) is a chronic demyelinating and neurodegenerative disease of the central nervous system (CNS). Although the etiology of MS is not completely understood, there is evidence suggesting a role for infectious agents in the pathophysiology of MS. There are evidences of a possible association between MS and Chlamydia pneumonia (CPn), however a consensus on this issue...