Small RNAs (21-24 nucleotides) including microRNAs (miRNAs) and small interfering RNAs (siRNAs) are potent regulators of gene expression in both plants and animals. Several hundred genes encoding miRNAs and thousands of siRNAs have been experimentally identified by cloning approaches. New sequencing technologies facilitate the identification of these molecules and provide global quantitative ex...

Mucopolysaccharidoses (MPSs) are inherited lysosomal storage disorders (LSDs). MPSs caused by excessive accumulation of mucopolysaccharides due to missing or deficiency enzymes required for the degradation specific macromolecules. MPS I-IV, VI, VII, and IX sub-types mucopolysaccharidoses. Among these, III (also known as Sanfilippo) IV (Morquio) syndromes lethal prevalent sub-types. This study a...

as a high-throughput method to identify short transcripts devoid of poly-A tails, as microRNAs and sRNAs (Sunkar and Zhou, 2004). In September 2005, Science published a monograph on non-coding RNAs the first analysis of a large number of small transcribed Arabidopsis RNAs using MPSS (Lu et al., 2005). In this timeframe, findings on viroids and the movement of viral proteins through the phloem i...

SUMMARY We have developed U-PRIMER, a primer design program, to compute a minimal primer set (MPS) for any given set of DNA sequences. The U-PRIMER algorithm, which uses automatic variable fixing and automatic redundant constraint elimination to tackle the binary integer programming problem associated with the MPS selection problem. The program has been tested successfully with 32 adipocyte dev...

Exercise Paton was a New South Wales-wide simulation exercise conducted on 30 November 2006, to test the response of New South Wales emergency departments (EDs), multi-purpose services* (MPSs), and public health units to the presentation of single cases of pandemic influenza during the early stages of a pandemic. The exercise followed the release of the New South Wales policy document to guide ...

BACKGROUND A comprehensive network-based understanding of molecular pathways abnormally altered in glioblastoma multiforme (GBM) is essential for developing effective therapeutic approaches for this deadly disease. METHODOLOGY/PRINCIPAL FINDINGS Applying a next generation sequencing technology, massively parallel signature sequencing (MPSS), we identified a total of 4535 genes that are differ...

Tujuan dari penelitian ini adalah untuk mengetahui hubungan antara resiliensi dan dukungan sosial dengan stress akademik mahasiswa baru di Jakarta pada masa pandemi Covid-19. Metode yang digunakan metode korelasi. Sampel berjumlah 470 Universitas Negeri ditentukan teknik insidental. Pengumpulan data dilakukan menggunakan kuesioner the Connor-Davidson Resilience Scale (CD-RISC), Multidimensional...

Background and Objectives: The goal of current study is to evaluate the performance of hospitals and their departments. This manuscript aimed at estimation of the most productive scale size (MPSS), returns to scale (RTS), and benchmarking for inefficient hospitals and their departments. Methods: The radial and non-radial data envelopment analysis (DEA) ap...

Protein content in rice grain (Oryza sativa L.) is an important trait from human nutrition perspective, particularly for those having rice as a main food in daily life. Several QTLs identified for the grain protein content (GPC) needs refinement and further genetic dissection to truly understand the trait. In this study we have searched for the putative candidate genes underlying five known QTL...