نتایج جستجو برای: mouse embryonic fibroblast cell

تعداد نتایج: 1908287  

Journal: :Arteriosclerosis, Thrombosis, and Vascular Biology 2021

Objective: The superoxide-generating Nox2 (NADPH oxidase-2) is expressed in multiple cell types. Previous studies demonstrated distinct roles for cardiomyocyte, endothelial cell, and leukocyte ANG II (angiotensin II)–induced cardiovascular remodeling. However, the vivo role of fibroblast remains unclear. Approach Results: We developed a novel mouse model with inducible fibroblast-specific defic...

Journal: :مجله دانشکده پزشکی اصفهان 0
مرضیه موج بافان دانشجوی فوق لیسانس، گروه زیست شناسی، دانشکده ی علوم، دانشگاه اصفهان، اصفهان، ایران. کامران قائدی استادیار، گروه زیست شناسی، دانشکده ی علوم، دانشگاه اصفهان و پژوهشکده ی رویان، گروه زیست شناسی سلولی و مولکولی، مرکز تحقیقات علوم سلولی جهاد دانشگاهی، اصفهان، ایران. شهناز رضوی دانشیار، گروه آناتومی، دانشکده ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران. فرشته کرمعلی گروه سلول های بنیادی و زیست شناسی تکوینی، پژوهشکده ی رویان، مرکز تحقیقات علوم سلولی جهاد دانشگاهی، تهران، ایران. خدیجه کربلایی گروه سلول های بنیادی و زیست شناسی تکوینی، پژوهشکده ی رویان، مرکز تحقیقات علوم سلولی جهاد دانشگاهی، تهران، ایران. سمیه تنهایی گروه سلول های بنیادی و زیست شناسی تکوینی، پژوهشکده ی رویان، مرکز تحقیقات علوم سلولی جهاد دانشگاهی، تهران، ایران. فرزانه ربیعی

background: p19 cells are mouse embryonic carcinoma cells which contain pluripotent ability, like stem cells, to differentiate into different cell lines. there are several properties for this cell line that make it a valuable cell model for study of developmental stages. methods: at the first step, pts2-egfp coding sequence which was cloned in pucd2.hygro vector was used for transfection in to ...

حویزی, الهام, محمدی, طیبه,

Background: One of the major causes of death in the world is cancer and therefore any study in the field of cancer biology is of great importance. Head and neck cancers represent approximately 2-5% of neoplasms which is higher in some countries. The most appropriate therapy for various cancers is identifying effective and efficient ways that contribute to initiation of apoptosis. Cyclophosphami...

Journal: :Human Reproduction 2023

Abstract At the moment of union in fertilisation, sperm and oocyte are transcriptionally silent, but little is understood about how transcription initiates newly-formed embryo. In this talk, I shall suggest why fundamental process has remained arcane for so long, my lab sought to unlock its secrets mouse human embryos. By combining micromanipulation single-cell RNA-sequencing, we have revealed ...

Journal: :The Journal of Cell Biology 1981
M Mével-Ninio M C Weiss

We have used a combination of a sensitive immunocytochemical stain for intracellular albumin, and Hoechst 33258 dye for identification of parental nuclei to investigate the time-course of extinction, reexpression, and activation of albumin production in fusion products of 1s (hyperdiploid) or 2s (hypertetradiploid) rat hepatoma cells with mouse fibroblasts (L cells or embryonic cells). In all c...

Background: Recombination Activating Genes (RAG) mutated embryonic stem cells are (ES) cells which are unable to perform V (D) J recombination. These cells can be used for generation of immunodeficient mouse. Creating biallelic mutations by CRISPR/Cas9 genome editing has emerged as a powerful technique to generate site-specific mutations in different sequences. Ob...

Journal: :Frontiers in Materials 2021

In this study we compare the leaching behavior and antibacterial cytotoxic properties of 100% slag or stone wool derived alkali activated materials. The activity was measured as inhibiting capacity against two Gram-negative bacterial strains, Escherichia coli Pseudomonas aeruginosa one Gram-positive strain: Enterococcus faecalis . cytotoxicity were tested on mouse embryonic fibroblast NIH-3T3 c...

Background: Aging is one of the important factors in the development of age-related diseases. Acrylamide can be produced during carbohydrate-rich foods prepared at high temperatures. Recently, studies showed that acrylamide can induce cellular senescence. On the other hand, Rutin as a natural flavonoid, has a potent antioxidant activity. Objective: This study aims to evaluate the ptotective ef...

Journal: :Blood 2007
Linlin Wang Thomas C Schulz Eric S Sherrer Derek S Dauphin Soojung Shin Angelique M Nelson Carol B Ware Mei Zhan Chao-Zhong Song Xiaoji Chen Sandii N Brimble Amanda McLean Maria J Galeano Elizabeth W Uhl Kevin A D'Amour Jonathan D Chesnut Mahendra S Rao C Anthony Blau Allan J Robins

Despite progress in developing defined conditions for human embryonic stem cell (hESC) cultures, little is known about the cell-surface receptors that are activated under conditions supportive of hESC self-renewal. A simultaneous interrogation of 42 receptor tyrosine kinases (RTKs) in hESCs following stimulation with mouse embryonic fibroblast (MEF) conditioned medium (CM) revealed rapid and pr...

Journal: :Materials Chemistry and Physics 2021

Porous titanium alloy Ti6Al4V scaffolds manufactured via electron beam melting (EBM®) reveal broad prospects for applications in bone tissue engineering. However, local inflammation and even implant failure may occur while placing an into the body. Thus, application of drug carriers to surface a metallic can provide treatment at site. In this study, we propose use polyelectrolyte (PE) microcaps...

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