Cardiovascular disease is the most common cause of death in diabetic patients. Hyperglycemia is the primary characteristic of diabetes and is associated with many complications. The role of hyperglycemia in the dysfunction of human cardiac progenitor cells that can regenerate damaged cardiac tissue has been investigated, but the exact mechanism underlying this association is not clear. Thus, we...

Graft failure after liver transplantation may involve mitochondrial dysfunction. We examined whetherpreventionofmitochondrial injurywould improvegraft function.Orthotopic rat liver transplantationwas performed after 18 hours’ cold storage inUniversity ofWisconsin solution and treatment with vehicle, minocycline, tetracycline, or N-methyl-4-isoleucine cyclosporin (NIM811) of explants and recipie...

Dehydroepiandrosterone (DHEA) is widely used as a nutritional supplement and exhibits putative anti‑aging properties. However, the molecular basis of the actions of DHEA, particularly on the biological characteristics of target cells, remain unclear. The aim of the current study was to investigate the effects of DHEA on cell viability, cell proliferation, cell cycle and mitochondrial function i...

Preconditioning (PC) as a protective strategy against noxious insults can decline cell death and apoptosis. It has been approved that mitochondria play a key role in PC mechanism. The critical role of complex I (CI) in oxidative phosphorylation machinery and intracellular ROS production, particularly in the brain, accentuates its possible role in PC-induced neuroprotection. Here, differentiated...

Mitochondrial biogenesis is a cell response to external stimuli which generally believed suppress apoptosis. However, during the process of apoptosis, whether mitochondrial occurs in early stage apoptotic cells remains unclear. To address this question, we constructed COX8-EGFP-ACTIN-mCherry HeLa with recombinant fluorescent proteins respectively tagged on nucleus and mitochondria, monitored ch...

We examine the basis of Darwin's corollary to Haldane's rule, which describes viability and fertility differences between F1 produced from reciprocal crosses. We analyzed asymmetries in hybrid viability from >100 reciprocal crosses involving 36 toad species to test whether relatively high rates of mitochondrial vs. nuclear evolution produce dams with systematically less viable F1 hybrid progeny...

Cell viability may be judged by morphological changes or by changes in membrane permeability and/or physiological state inferred from the exclusion of certain dyes of the uptake and retention of others. This unit presents methods based on dye exclusion, esterase activity, and mitochondrial membrane potential, as well as protocols for determining the pre-fixation viability of fixed cells either ...