Using a modified radiolabeled primer extension method (we named this modification misGvA-"misincorporation of G versus A") we have investigated the DNA synthesis and repair at early and late stages of development of loach Misgurnus fossilis. The misincorporation activity of DNA polymerase iota (Pol ι) in wild-type loach could not be detected by this method at any stage of loach development. In ...

Guizhou is an important ecological barrier in the upper reaches of Yangtze River and Pearl basins with abundant fish species. However, from these regions are threatened by anthropogenic activities, including overfishing habitat destruction. Here, we assessed diversity more than half species region using DNA barcoding (partial sequence cytochrome c oxidase subunit I (COI) gene). We obtained 800 ...

MicroRNAs (miRNAs) exert important roles in animal growth, immunity, and development, and regulate gene expression at the post-transcriptional level. Knowledges about the diversities of miRNAs and their roles in accessory air-breathing organs (ABOs) of fish remain unknown. In this work, we used high-throughput sequencing to identify known and novel miRNAs from the posterior intestine, an import...

The problem of osmoregulation in teleosts has been extensively studied by a number of workers to the effect that the basic plan of adaptive water and salt balance is figured out in its outline(Krogh, 1939; Baldwin, 1949; Black, 1951; Gaunt and Birnie, 1951; Fontaine, 1953, etc.). Several lines of evidences are available for physiological interpretation of observed osmotic phenomena in terms of ...