Toxicity screening of compounds provides a means to identify compounds harmful for human health and the environment. Here, we further develop the technique of genomic phenotyping to improve throughput while maintaining specificity. We exposed cells to eight different compounds that rely on different modes of action: four genotoxic alkylating (methyl methanesulfonate (MMS), N-Methyl-N-nitrosoure...

Carotid body (CB) chemoreceptor cells detect physiological levels of hypoxia and generate a hyperventilation, homeostatic in nature, aimed to minimize the deleterious effects of hypoxia. Intimate mechanisms involved in oxygen sensing in chemoreceptor cells remain largely unknown, but reactive oxygen species (ROS) had been proposed as mediators of this process. We have determined glutathione lev...

We have previously reported that the reducing agent dithiothreitol (DTT) strongly increases thermally induced activity of the transient receptor potential vanilloid receptor-1 (TRPV1) channel. Here, we show that exposure to oxidizing agents also enhances the heat-induced activation of TRPV1. The actions of sulfhydryl modifiers on heat-evoked whole-cell membrane currents were examined in TRPV1-t...

this study describes the organic synthesis of 5-(2-amimo-4-styryl pyrimidine-4-yl)-4-methoxy benzofuran-6-ol (spbf) as an example of a benzofuran derivative used as a new series of amyloid imaging agents. these benzofuran derivatives may be useful amyloid imaging agents for detecting b-amyloid plagues in the brain of alzheimer’s disease. the precursor is 1-[6-hydroxy-4-methoxybenzofuran-5-yl]-p...

Preformulation studies regarding a physico-chemical evaluation of memantine, a non-competitive N-methyl-D-aspartate (NMDA) receptor antagonist, are presented in this work. This characterization of memantine is pre-required for the formulation of a parenteral solution. The water solubility, the thermal behavior and the chemical stability in aqueous solution, depending on the pH, temperature, UV ...

The textile industry is one of the major sources environmental pollution. This due to use a wide range dyes, surfactants, oxidizing agents and other chemical reagents in technological processes. Modern dyes are characterized by low biodegradability, resistance temperature effects environment. Therefore, development cost-effective effective measures combat such pollution necessary protect ecosys...