During the course of a mechanism-based screening program designed to identify new microtubule-disrupting agents from natural products, we identified a crude extract from Tacca chantrieri that initiated Taxol-like microtubule bundling. Bioassay-directed purification of the extract yielded the highly oxygenated steroids taccalonolides E and A. The taccalonolides caused an increased density of cel...

The irregularity of growing and shortening patterns observed experimentally in microtubules their ensembles reflects a dynamical system that fluctuates stochastically between assembly disassembly phases. time series microtubule lengths have been extensively analyzed to shed light on structural properties microtubules. Here, for the first time, Multifractal Detrended Fluctuation analysis (MFDFA)...

BACKGROUND & AIMS Secretin induces bicarbonate-rich hydrocholeresis in healthy individuals, but not in untreated patients with primary biliary cirrhosis (PBC). Ursodeoxycholic acid (UDCA)--the first choice treatment for PBC--restores the secretin response. Compared with humans, secretin has poor effect in experimental normal-rat models with biliary drainage, although it may elicit hydrocholeres...

Background Globally, a number of individuals who live with Alzheimer’s disease (AD), an incurable and debilitating disease, is increasing. Tau protein has structural role, maintains integrity microtubules one the underlying causes tauopathies [1]. It unfolded highly soluble under physiological conditions. Dopamine receptor agonists have been used to target motor symptoms AD [2] but there gap in...

Drugs that target microtubules are potent inhibitors of angiogenesis, but their mechanism of action is not well understood. To explore this, we treated human umbilical vein endothelial cells with paclitaxel, vinblastine, and colchicine andmeasured the effects onmicrotubule dynamics and cellmotility. In general, lower drug concentrations suppressedmicrotubule dynamics and inhibited cell migratio...

Kinesin motor proteins comprise an ATPase superfamily that works hand in hand with microtubules in every eukaryote. The mitotic kinesins, by virtue of their potential therapeutic role in cancerous cells, have been a major focus of research for the past 28 years since the discovery of the canonical Kinesin-1 heavy chain. Perhaps the simplest player in mitotic spindle assembly, Kinesin-5 (also kn...

Actin polymerization is a universal mechanism to drive plasma membrane protrusion in motile cells. One apparent exception to this rule is continuing, or even accelerated outgrowth of neuronal processes in the presence of actin polymerization inhibitors. This fact together with a key role of microtubule dynamics in neurite outgrowth led to the concept that microtubules directly drive plasma memb...

In this paper, we use the modified exp−ψθ-function method to observe some of solitary wave solutions for microtubules (MTs). By treating issues as nonlinear model partial differential equations describing microtubules, were able solve problem. We then found specific evolution equation (NLEE) covering various parameters that are particularly significant in biophysics and nanobiosciences. additio...

Cell stiffening induced by anticancer microtubule targeting drugs (MTDs) such as vinflunine (VFL), colchicine (COL), and docetaxel (DTX) can be driven crosstalk between actin filaments microtubules.