Subfractionation of Microsomes, Cell Suspension Culture, PAL, Cinnamic Acid p-Hydroxylase 1. The time course of activity of soluble and microsomal phenylalanine ammonia-lyase (PAL) was studied in dark grown cell cultures of soybean (Glycine max). A distinct activity increase of PAL in the soluble and microsomal fraction occurred prior to the stationary phase of the cell culture. Cinnamic acid p...

The turnover of various constituents of the membranes of the endoplasmic reticulum has been measured by injecting 14C-leucine and W-acetate, or in some cases 14C-leucine and 14C-glycerol, into a series of 12 to 15 rats (weighing 150 to 200 g). At various times, up to 2 weeks after injection, three rats were killed, their livers were pooled, microsomes were obtained and divided into rough and sm...

Purified rat liver ATP citrate lyase is shown to bind to the microsomal fraction of rat liver. Under the same conditions the enzyme does not bind significantly to the mitochondrial fraction or to the outer membrane prepared from the mitochondrial fraction. The binding component of the microsomal fraction is further identified as the endoplasmic reticulum, and a protein component of the membrane...

Peroxynitrite (ONOO-) toxicity is associated with protein oxidation and/or tyrosine nitration, usually resulting in inhibition of enzyme activity. We examined the effect of ONOO- on the activity of purified rat liver microsomal glutathione S-transferase (GST) and found that the activity of reduced glutathione (GSH)-free enzyme was increased 4- to 5-fold by 2 mM ONOO-; only 15% of this increased...

PURPOSE We investigated a potential hepatoprotective role of d-cis diltiazem, l-cis diltiazem, thiamine and the combination d-cis diltiazem and thiamine against lipid peroxidation in a piglet liver microsomal model. A modified in vitro dichlorofluorescein assay was developed to assess the extent of peroxidative damage induced by reactive oxygen species in the piglet liver microsomal fraction. ...

Purified Signal Recognition Protein (SRP) has previously been shown to be required for the translocation of secretory proteins across the microsomal membrane (Walter and Blobel, 1980. Proc. Natl. Acad. Sci. U. S. A. 77:7, 112-7, 116) and to function in the early events of this process (Walter and Blobel, 1981. J. Cell Biol. 91:557-561). We demonstrate here that the delta subunit of acetylcholin...

The rates of synthesis, turnover, and half-lives were determined for brain microsomal ether phospholipids in the awake adult unanesthetized rat. A multicompartmental kinetic model of phospholipid metabolism, based on known pathways of synthesis, was applied to data generated by a 5 min intravenous infusion of [1,1-(3)H]hexadecanol. At 2 h post-infusion, 29%, 33%, and 31% of the total labeled br...

Z. Naturforsch. 50c, 29-36 (1995); received August 29/September 30,1994 Cucumis (Germination), Integral Membrane Protein, Isoenzyme (Lipoxygenase), Lipoxygenase, Methyl Jasmonate, Plasma Membrane In cucumber cotyledons (Cucumis sativus L.) containing several soluble and particulate forms of lipoxygenase (LOX), the location of LOX forms in microsomes has been studied. We concentrated on the ques...

s of Communications Meeting of the Biochemical Society Pharmacological Biochemistry Group, St. Mary3 Hospital Medical School, London 13 September 1974 GLUCURONIDATION MECHANISMS : a Colloquium organized by P. T. Nowell (Liverpool) Membrane Dependence of Uridine Diphosphate Glucuronyltransferase : Effect of the Membrane on Kinetic Properties DAVID ZAKIM and DONALD A. VESSEY Departments of Medici...

The effects of purified Helminthosporium maydis T (HmT) toxin on active Ca(2+) transport into isolated mitochondria and microsomal vesicles were compared for a susceptible (T) and a resistant (N) strain of corn (Zea mays). ATP, malate, NADH, or succinate could drive (45)Ca(2+) transport into mitochondria of corn roots. Ca(2+) uptake was dependent on the proton electrochemical gradient generated...