a thirteen-year-old girl from dezfoul, iran was referred to our hospital with a history of eight days of high fever, headache, odynophagia, diffuse abdominal and body pain especially limb pain. she then developed conjunctival erythema, transient maculopapular rash on the trunk and also diplopia. no obvious or specific point was shown in the history. in the lab studies, she had very high sedimen...

INTRODUCTION Cart horses are a re-emerging population employed to carry recyclable material in cities. METHODS Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed. RESULTS A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. B...

Serum samples collected from Iberian red deer (Cervus elaphus hispanicus; n=472), fallow deer (Dama dama; n=293) and European wild boar (Sus scrofa; n=174) in Asturias, Northern Spain, from 1999 to 2005 were examined for antibodies against a reference panel of 14 Leptospira spp. serovars. Positive antibody titres at a microscopic agglutination test cut-off of 1:80 were detected against serovars...

INTRODUCTION Leptospirosis is caused by a bacterium of the genus Leptospira. This study aimed at investigating the seroprevalence of and risk factors for leptospirosis in humans in Manaus, State of Amazonas. METHODS Interviews were performed, and 1,000 blood serum samples were examined using a microscopic agglutination test. RESULTS Forty-three cases were positive; there were 10 serotypes, ...

Methods Retrospective study, between January 2004-June 2014, in one infectious diseases hospital in Bucharest. We included patients with leptospirosis diagnosis at discharge and/or positive serology for leptospirosis. Serological diagnosis of acute leptospirosis was made by microscopic agglutination test using a battery of 17 antigens from international reference strains in one national referen...

A total of 400 serum samples collected from patients, clinically suspected of leptospirosis, were evaluated for antibodies by LEPTO dipstick and microscopic agglutination test (MAT). Twenty of these patients (5%) had serological evidence of leptospirosis. Leptospira interrogans serovars Autumnalis and Icterohaemorrhagiae, Canicola and Javanica were serogroups recorded serologically. Fever and j...

Aim: To study the seroprevalence of leptospirosis in dogs in Kerala and to identify the most prevalent serovar. Materials and Methods: A total of 205 sera collected from dogs were screened for the presence of antibodies against leptospirosis by Microscopic Agglutination Test (MAT). Results: A seroprevalence rate of 71.12 per cent was observed. Leptospira interrogans serovar Autumnalis was found...

A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positiv...

We describe the search toward a fast and reliable strategy to detect and confirm the presence of methicillin-resistant Staphylococcus aureus (MRSA) in screening samples. First, we evaluated the sensitivities and specificities of oxacillin resistance screening agar (ORSA) with enrichment (tryptic soy broth [TSB] and ORSA [TSB-ORSA]) and without enrichment (ORSA), MRSA ID (MRSA_ID) plates, and CH...