A simple micropropagation protocol has been achieved using nodal region and apical leaf of Clerodendrum indicum,(L) kuntze; an important medicinal plant of Verbenaceae. The explants were cultured in MS medium supplemented with NAA, BA, 2,4-D,Kn, IBA in various combination and concentrations (0.110.0 μM). Response to callogenesis were diverse, ranging from white vigorous compact callus (1.0μM NA...

Operculina turpethum (syn. Ipomoea turpethum) a plant in the morning glory family is an endangered medicinal plant of Bangladesh and its neighboring countries. An efficient micropropagation protocol was established for this plant through nodal segment culture as well as antimicrobial activity of the leaves was also evaluated. For micropropagation, nodal segments with axillary buds of field grow...

Genetic variation due to somaclonal variation in micropropagated plants is a beneficial phenomenon for crop improvement. Genetic integrity of the plants derived through micropropagation becomes crucial if genetic transformation studies have to be carried out. Somaclonal variation in tissue culture is a common phenomenon which makes it mandatory to check for genetic stability of plants. Hypocoty...

In rubber, the current propagation method of grafting on to unselected seedlings, maintains intraclonal heterogeneity for vigour and productivity and hence a great improvement may be expected by using micropropagation in vitro. Micropropagation with nodal and shoot tip explants derived from seedlings are possible as with mature clonal explants. The major problem in using clonal material from ma...

The micropropagation of Lilium longiflorum requires adequate equipment which may not be afforded by small laboratories or producers. In this work we compared traditional methodology with a protocol that included easily available elements to sterilize materials and culture media, together with addition of hydrogen peroxide (H2O2) into the nutrient media as chemical sterilizer. A series of H2O2 c...