Five water samples from three sources, two municipal reservoirs in central North Carolina and Toolik Lake in Alaska, were processed to conduct a comparative survey of microbial small subunit rDNA sequences. Genomic DNA was extracted and amplified by PCR using universal SSU rDNA primers to generate 16S and 18S rDNA clone libraries and 50 clones from each library were sequenced and placed in oper...

Ecological restoration of mine tailings have impact on soil physiochemical properties and microbial communities. The surface soil has been a primary concern in the past decades, however it remains poorly understood about the adaptive response of microbial communities along the profile during ecological restoration of the tailings. In this study, microbial communities along a 60-cm profile were ...

Using soil molecular biology to make a traceable fingerprint has been proposed as putative method for forensic science. One unknown of this method is how the soil microbial community responds to contrasting conditions for storage and handling. To examine this we compared field fresh sample to soil samples stored one of six ways (4 ̊C, -20 ̊C, -80 ̊C, air dried, freeze dried, and oven dried) for 5 ...

Background and Objectives: Infection of pleural fluid is a common disease and because of antibiotic administration, the microbiology of this fluid has changed. The aim of this study was to determine the common bacteria and suitable antibiotics for treatment in pleural effusion (PE). Materials and Methods: In this cross sectional study, 1210 samples with exudative features were cultured for poss...

MOTIVATION A number of community profiling approaches have been widely used to study the microbial community composition and its variations in environmental ecology. Automated Ribosomal Intergenic Spacer Analysis (ARISA) is one such technique. ARISA has been used to study microbial communities using 16S-23S rRNA intergenic spacer length heterogeneity at different times and places. Owing to erro...

OBJECTIVE Urease enzymes produced by oral bacteria generate ammonia, which can have a significant impact on the oral ecology and, consequently, on oral health. To evaluate the relationship of urease with dental plaque microbial profiles in children as it relates to dental caries, and to identify the main contributors to this activity. METHODS 82 supragingival plaque samples were collected fro...

Site U1317 (proposal Site PORC-3A) is located on the northwest shoulder of the Challenger Mound (51o 22.8 N, 11o 43.1W, in 781 to 815 m water depth). Scientific drilling of the Challenger Mound was the central objective of Expedition 307. Specific objectives of drilling Site U1317 were: • To establish whether the mound base is on a carbonate hardground of microbial origin and whether past geofl...