background the 30 kda major secretory protein of mycobacterium tuberculosis (antigen 85b) is a primary vaccine candidate. this secreted antigen induces a protective immune response and stimulates the production of ifn-γ in animal models. objectives the aim of this study was cloning and expression of ag 85b of m. tuberculosis in escherichia coli. materials and methods to produce recombinant ag85...

Brief heating of Bacillus Licheniformis cell walls at 100 degrees C in aqueous buffers of pH3.0-4.0 removes some polymers but not others from the mucopeptides. For example, relatively undegraded teichuronic acid can be extracted at 100 degrees C in 20min at pH3.0 whereas the teichoic acids are not removed. Similar specificity can be shown with walls from three other species of micro-organism.

Mycoplasma hominis is a fastidious micro-organism causing genital and extragenital infections. We developed a specific real-time PCR that exhibits high sensitivity and low intrarun and interrun variabilities. When applied to clinical samples, this quantitative PCR allowed to confirm the role of M. hominis in three patients with severe extragenital infections.

Cycloheximide resistance in Tetrahymena is inducible by mutagenesis. Two mutations, isolated independently from genetically different strains, were analyzed. The two mutations, Chx-1 and Chx-2, were found to be alleles at the same locus. A specific analysis is presented which may serve as a model for strategies employing an independent manipulation of the micro-and macronuclei of this organism ...

from his investigations that the relapsing fever of Panama is distinct from the analogous fever of Africa Europe and Asia, although belonging to the same class. The micro-organism causing | the local relapsing fever belongs to the group Sp. obermeieri, Sp. duttoni, and bp. carten. lhe natural mode of infection has not been definitely established so far. Sarcosporidiosis.?(With Report of a case 111

Introduction: Tracheostomy is a life saving emergency procedure and provides many benefits for long term ventilator dependent patients. However, colonization and infection of airways after tracheostomy is one of the serious complications increasing patient morbidity and mortality. Objective: To find out the colonizing agent in the trachea of tracheostomized patients and the type and frequency o...

This paper develops a two-gene, single fitness peak model for determining the equilibrium distribution of genotypes in a unicellular population which is capable of genetic damage repair. The first gene, denoted by sigma(via), yields a viable organism with first-order growth rate constant k>1 if it is equal to some target "master" sequence sigma(via,0). The second gene, denoted by sigma(rep), yi...

Since many micro-organisms are a biological hazard, they have been categorised into risk groups by many countries and organisations and classification lists have been developed. Current classification systems rely on criteria defined by the World Health Organization, which cover the severity of the disease the micro-organism might cause, its ability to spread and the availability of prophylaxis...

OBJECTIVE To assess the most common micro-organisms causing odontogenic infections and their antimicrobial susceptibility. METHODS The study was conducted in 80 patients with orofacial infection. The pus sample was collected, cultured (aerobically and anaerobically) and stained for morphological study of the isolates. Antibiotic sensitivity test for the isolates were performed. RESULTS A to...

Normal human serum contains a few hundred micro-microgrammes (,u,ug.) of vitamin B12 per ml. A micro-microgramme is one-millionth of a millionth of a gramme, or I/I,000,000,000 of a milligramme. No chemical method is capable of measuring such small amounts. Specialized techniques are necessary and, with one exception, all methods employ microbiological assays. Certain micro-organisms require vi...