We have developed two novel schemes for the direct selection of peroxisome-biogenesis-defective (pex) mutants of the methylotrophic yeast Pichia pastoris. Both schemes take advantage of our observation that methanol-induced pex mutants contain little or no alcohol oxidase (AOX) activity. AOX is a peroxisomal matrix enzyme that catalyzes the first step in the methanol-utilization pathway. One sc...

Exploring new fields: Lanthanide-using bacteria that turn over methane or methanol in their metabolism can replace essential lanthanides by certain actinides. In Research Article (e202303669), Lena J. Daumann et al. show an extremophile isolated from a volcanic mud-pot as well methylotrophic laboratory strain use americium and curium. vivo growth experiments vitro studies with the key metabolic...

This discussion will highlight the following 9 specific points that related to metabolic acidosis caused by various toxins. The current recommendation suggests that alcohol dehydrogenase inhibitor fomepizole is preferred to ethanol in treatment of methanol and ethylene glycol poisoning, but analysis of the enzyme kinetics indicates that ethanol is a better alternative. In the presence of a mode...

Abstrsct—An enzyme system capable of oxidizing both methanol and ethanol in the 0 presence of DPN was purified almost nine ty-fold from the liver of the rhesus monkey. Data for theK, values for the two alcohols and DPN and for the R values in the presence of some inhibitors are presented. The enzyme ap rs to be identical to liver alcohol hydrogenase AD 1. Eihlili competitively inhibited the oxi...

Abstract ?-Alanine (3-aminopropionic acid) is the only naturally occurring ?-amino acid and an important precursor for synthesis of a variety nitrogen-containing chemicals. Fermentative production ?-alanine from renewable feedstocks such as glucose has attracted significant interest in recent years. Methanol become emerging promising feedstock biomanufacturing alternative to glucose. In this wo...

methanol is responsible for a life-threatening poisoning. fomepizole, a potent alcohol dehydrogenase inhibitor, is an efficient and safe antidote that prevents or reduces toxic methanol metabolism. although no study has compared its efficacy with ethanol, fomepizole is recommended as a first-line antidote. treatment should be started as soon as possible, based on history and initial findings in...

The crystal structure of methanol dehydrogenase (MDH) from Methylobacterium extorquens has been refined without stereochemical restraints at a resolution of 1.2 A. The high-resolution data have defined the conformation of the tricyclic pyrroloquinoline quinone (PQQ) cofactor ring as entirely planar. The detailed definition of the active-site geometry has shown many features that are similar to ...

A mutant of Methylobacterium extorquens AM1 with lesions in genes for three formate dehydrogenase (FDH) enzymes was previously described by us (L. Chistoserdova, M. Laukel, J.-C. Portais, J. A. Vorholt, and M. E. Lidstrom, J. Bacteriol. 186:22-28, 2004). This mutant had lost its ability to grow on formate but still maintained the ability to grow on methanol. In this work, we further investigate...