The presence of catalytic metal ions in RNA active sites has often been inferred from metal-ion rescue of modified substrates and sometimes from inhibitory effects of alternative metal ions. Herein we report that, in the Tetrahymena group I ribozyme reaction, the deleterious effect of a thio substitution at the pro-Sp position of the reactive phosphoryl group is rescued by Mn2+. However, analys...

Designed metal ion binding peptides offer a variety of applications in both basic science as model systems of more complex metalloproteins, and in biotechnology, e.g. in bioremediation of toxic metal ions, biomining or as artificial enzymes. In this work a peptide (HS: Ac-SCHGDQGSDCSI-NH2) has been specifically designed for binding of both Zn(II) and Hg(II), i.e. metal ions with different prefe...

Effects of Pb and several other metal ions on various distinct types of voltage-, receptor- and Ca-activated ion channels have been investigated in cultured N1E-115 mouse neuroblastoma cells. Experiments were performed using the whole-cell voltage clamp and single-channel patch clamp techniques. External superfusion of nanomolar to submillimolar concentrations of Pb causes multiple effects on i...

5.3. Xenobiotic Trace Metals and Materials 4804 5.3.1. Toxicology of Heavy Metals 4804 5.3.2. Nanoparticle Applications 4810 5.4. Environmental Metal Speciation 4812 5.4.1. Bacteria 4812 5.4.2. Aquatic Organisms 4812 6. Mass Spectrometry Imaging Techniques 4813 6.1. Secondary Ion Mass Spectrometry (SIMS) 4813 6.1.1. Metal Ion Distribution in Chromosomes 4813 6.1.2. Subcellular Distribution of M...

As the gravity of glycoscience continues to amass, a commensurate demand for rapid, sensitive, and structurally comprehensive glycoanalytical tools has arisen. Among the most elusive but desirable analytical capabilities is the ability to expeditiously and unambiguously detect, distinguish, and resolve carbohydrates that differ only in their constitutional isomerism and/or stereoisomerism. Whil...

bioremediation of heavy metals remains a major challenge in environmental biotechnology. biosorption is a novel technique for decreasing metal ion content in the waste water. biosorption experiments for cu (ii) were investigated in this study using bacteria isolated from the industrial effluent. the results showed that the dead cells of pseudomonas sp. were an efficient adsorbent of cu (ii). fo...

phytoremediation is an eco-friendly method for removal of pollutants, which can be relied upon as a sustainable technology, if implemented under optimum conditions of plant growth. the effectiveness of water hyacinth, a topical weed, for the removal of zinc (zn) and chromium (cr) ions from aqueous solutions has been presented in this article. the potential of this plant in removing metals by ph...

The reliability of different electrode admittance equations in the case of the Ag/Ag I electrode is critically discussed. It is shown that the admittance equation already derived for the' metal arnalgam/metal-Ion reversible electrode reaction coupled with reactant adsorption offers some advantages compared to the admittance equation for the reversible electrode reaction on the metal/metal-ion e...

The aim of this study was to remove Cd by ion-exchange resin Amberjet 1200H in flow condition. The adsorption characteristics of metal onto the resin were accurately described by Freundlich isotherms. The ion exchange of cadmium on resin follows pseudo-second-order kinetics. Ion exchange capacities of the cadmium on the ion exchange resin was studied in metal solution. The capacities of sorptio...