We have recent,ly determined the t,otal amino acid sequence of an extracellular nuclease of Staphylococcus aureus consisting of a single chain of 149 residues devoid of half-cystinelf 2 (see Fig. 1). This globular protein contains some regions of helical structure,3 can undergo completely reversible unfolding in a variety of denaturing solvents,4 and readily yields cryst’als suitable for X-ray ...

Functionalized chiral diazaphospholanes ligate to a variety of transition metals, yielding chiral, catalytically active, metal complexes. Previous work has established that amino acid derivatization of the carboxyl groups of (R,R)-N,N'-phthaloyl-2,3-(2-carboxyphenyl)-phenyl-3,4-diazaphospholane (1) yields phosphines that are excellent ligands for palladium-catalyzed asymmetric allylic alkylatio...

Synthetic alphaT1 and betaT1, the N-terminal tryptic peptides of alpha-chain and beta-chain of hemoglobin, and MetalphaT1 and MetbetaT1, peptides in which N-terminal methionyl residues are peptide-bonded to alphaT1 and betaT1, were prepared by the solid-state method of Merrifield. These synthetic peptides were used to establish conditions for chromatographic purification and analysis. When tryp...