SKG or BALB/c T cells (3.0 £ 10) were stimulated for 72 h with plate-bound anti-CD3 mAb (2C11) in the presence or absence of plate-bound anti-CD28 mAb in RPMI-1640 medium supplemented with 10% fetal calf serum and 50 mM 2-mercaptoethanol. Cells were also stimulated with TPA (1.4 ng ml) and ionomycin (0.14 mM). KJ1.26þ T cells from DO or DO.SKG mice were stimulated with ovalbumin (323–339) pepti...

conclusions with our laboratory diagnostic method, it seems that there are no cases of oci in patients with aih. however, we recommend further studies with more samples and more precise laboratory method. results six males and 29 females with mean disease duration of 77.1 ± 39.5 month and mean age of 43.62 ± 12.67 years were investigated. all cases were negative for anti-hcv antibody and we cou...

In this report, we examine the adaptability of commercially available serological kits to detect antibodies markers for viral hepatitis in oral fluid samples. We also assessed the prevalence of hepatitis A, B, and C virus-specific antibodies, and related risk factors for these infectious diseases through sensitivity of the tests in saliva samples to evaluate if oralfluid can be an alternative t...

Background and Aims: Occult hepatitis B virus infection (OBI) is known as an important source of hepatitis B virus (HBV) infection. It is categorized as Hepatitis B surface antigen (HBsAg) not being present and low DNA viral load in serum. In this study, an attempt was made to investigate the outbreak of anti-HBc and OBI among the HBsAg-negative donors in Golestan province. Materials and Metho...

Samples of human plasma having lipoprotein(a) (Lp[a]) protein levels between 5 and 15 mg/dl and a single apolipoprotein(a) (apo[a]) isoform were incubated in vitro at pH 7.7 with various concentrations (1-20 mM) of N-acetylcysteine, homocysteine, 2-mercaptoethanol (2ME), and dithiothreitol (DTT) for 1 hour at 37 degrees C under a nitrogen atmosphere. Sodium dodecyl sulfate-polyacrylamide gel el...

UNLABELLED We evaluated the sensitivity for the anti-HIV antibody detection of the 8 rapid disposable tests (RDTs) EC approved in 2008. METHODS A panel of 100 native serum samples collected from HIV-1 subtype B or non-B, HIV-1 group O or HIV-2-infected patient and 3 commercial seroconversion panels were tested. 50 sera from HIV-negative patients were included to blind reading and RDT specific...

BACKGROUND Occult hepatitis B virus (HBV) infection is characterized by the detection of HBV DNA in serum and/or in liver in the absence of detectable hepatitis B surface antigen (HBsAg). The reported prevalence of occult hepatitis B varies markedly among populations and according to the sensitivity of the HBV DNA assay. The aim of the present study was to describe the prevalence of occult hepa...

By means of the indirect haemagglutination test (IHAT), blood serum antibody titres against Toxoplasma gondii were analyzed in 380 sheep and 364 goats originating from 10 settlements in the Stara Zagora region, Bulgaria. Positive samples (titres > 1:80) were investigated one more time after treatment with 2-mercaptoethanol to detect IgM antibodies. In sheep, 183 samples (48.2 %) were positive, ...

The various tests for anti-double-stranded DNA antibodies do not always agree. Plasma fibronectin specifically binds DNA, is a component of immune complexes, and shows variations in concentration with disease activity in systemic lupus erythematosus. It may therefore interfere with the detection of DNA autoantibodies. This possibility was examined in a series of studies using the Crithidia luci...

The objective of the study was to evaluate the sensitivity, specificity and the positive and negative predictive value of a PCR assay for canine brucellosis diagnosis using 16S rRNA specific primers compared to serology, 2 mercaptoethanol-microtiter plate agglutination tests (2ME-MPAT) and a blood culture test. A sample of 48 dogs was divided into three groups, according to the results of blood...