Antibody formation in high-altitude-acclimatized and nonacclimatized rabbits was investigated at altitudes of 14,100 (Pikes Peak, Colo.) and 5,330 (Denver, Colo.). Serum pools representing samplings of primary response were titered before and after treatment with 2-mercaptoethanol. Peak titers of 2-mercaptoethanolresistant and -sensitive humoral antibodies were higher in the animals adapted to ...

Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losses could be estimated at 5-32% depending on polarities and concentrations. Results could be dramat...

Mouse peritoneal macrophages cultivated in vitro acquire a strong extracellular cytotoxic activity towards isotope labeled syngeneic erythrocytes as demonstrated by isotope release to the medium. This lytic process is mediated by an extremely labile macrophage cytolytic factor (MCF) which is not detected under ordinary tissue culture conditions with serum present in the medium. By the use of se...

Considerable interest has been expressed in recent years in the relative effects of different methods of DNA isolation on the physical, chemical, and biological properties of the material isolated. In particular, interest has been centered on methods of disrupting cells. Of these methods, mechanical abrasion, sonication, and chemical lysis have been most often reported. The effectiveness of mec...

Protein synthesis by a rabbit reticulocyte lysate is inhibited by the haemagglutinating lectins from Momordica charantia and Crotalaria juncea seeds and from the roe of Rutilus rutilus, and by a commercial preparation of the mitogenic lectin from Phytolacca americana. The haemagglutinins from the seeds of Ricinus communis and of Vicia cracca acquired inhibitory activity after their reduction wi...

The reliability of gene expression profiling based technologies and methods to find transcriptional differences representative for the original samples is influenced by the quality of the extracted RNA. Hence, RNA extraction is the first step to investigate the gene expression and its function. Consequently, the quality of extracted RNA is really significant. Correspondingly, this research was ...

the reliability of gene expression profiling based technologies and methods to find transcriptional differences representative for the original samples is influenced by the quality of the extracted rna. hence, rna extraction is the first step to investigate the gene expression and its function. consequently, the quality of extracted rna is really significant. correspondingly, this research was ...

objective: mesenchymal stem cells (mscs) can differentiate into various cell types. since cysteine has structural similarities to neuronal inducers β-mercaptoethanol and glutathione, we examined its effect on neural induction of rat bone marrow mscs. materials and methods: in this experimental study, cells were treated in a medium containing 1mm cysteine for 24 hours prior to treatment with neu...