MOTIVATION Realistic simulation of the kinetic properties of metabolic pathways requires rate equations to be expressed in reversible form, because substrate and product elasticities are drastically different in reversible and irreversible reactions. This presents no special problem for reactions that follow reversible Michaelis-Menten kinetics, but for enzymes showing cooperative kinetics the ...

A chitinase-producing bacteria was locally isolated from rhinoceros beetle (Oryctes rhinoceros) larvae gut discovered in mushroom compost. strain selected the source and labeled as RBLG1. It identified using 16s rDNAsequencing has strong homology to Bacillus cereus. Chitinase produced RBLG1 purified ammonium sulfate precipitation, ion exchange chromatography, gel filtration. The chitinase shows...

The use of enzymes to catalyze chemical reactions has increased these recent years. Several models have been developed express the kinetics over biocatalysts. most well-known them, Michaelis-Menten, is used when only one substrate adsorbs on enzyme. In case esterification reaction, i.e., bimolecular system, a more complex kinetic model such as Ping-Pong Bi-Bi should be applied. advanced essenti...

Rhus chinensis Mill is a potential plant for phytoremediation of Pb and able to uptake copious amount Pb. However, little known about the pathways properties in this plant. Here, controlled experiments were used assess translocation R. chinensis. The whole time-kinetics divided into two stages: rapid slower accumulation, processes fitted with linear model. concentration-dependent kinetics chara...

We provide initial rate data from enzymatic reaction experiments and tis processing to estimate the kinetic parameters from the substrate uncompetitive inhibition equation using the median method published by Eisenthal and Cornish-Bowden (Cornish-Bowden and Eisenthal, 1974; Eisenthal and Cornish-Bowden, 1974). The method was denominated the direct linear plot and consists in the calculation of ...