Thin sections of glutaraldehyde-fixed, epoxy resin-embedded bone marrow from rats were treated with 2% palladium chloride in 2% concentrated HCl. This procedure was found to induce high electron density in chromatin from all cell types and in cytoplasmic granules of neutrophils and eosinophils. In the latter, the crystalline body showed more contrast than the matrix.

Intrinsically disordered proteins (IDPs) can protect cells from diverse stresses by forming higher order assemblies such as reversible aggregates or granules. Recently, Boothby et al. show that IDPs protect tardigrades against desiccation by forming a glass-like amorphous matrix, highlighting that material properties of disordered proteins can confer adaptation during stress.

Motility initiation is a key event during internal fertilization of female-stored sperm, although the underlying mechanisms remain unclear. In internally fertilizing urodeles, quiescent sperm initiate motility on the surface of the egg-jelly, a thick extracellular matrix that accumulates around the egg in oviduct. By immunizing mice with egg-jelly extracts, we successfully generated an alpha34 ...

The perivitelline space of mammalian oocytes changes in size and composition during preimplantation development. Often overlooked in the past, this space contains a hyaluronan-rich extracellular matrix prior to fertilization and a cortical granule envelope following release of the cortical granules at fertilization. The hyaluronan-containing matrix of unfertilized oocytes is well developed in s...

Eggs of most animal species are fertilized by a single sperm and fusion of these two cells is essential for the activation of the developmental program of the zygote. Cases in which multiple sperm fertilize an egg, a condition known as polyspermy, the supernumerary paternal centrioles and chromosomes usually create aberrant cell divisions that result in death. Thus, eggs have evolved mechanisms...

We have studied a murine tumor previously classified as a poorly differentiated chondrosarcoma. Although the cells in this tumor are surrounded by large quantities of extracellular matrix material, the matrix fails to react with stains specific for the sulfated glycosaminoglycans present in normal cartilage. Here we show at the ultrastructural level that the tumor matrix is a homogeneous, nonfi...

SYNOPSIS. Electron microscopic observations of the fine structure and ontogeny of melanin granules in various inbred strains of mice have provided new information about the origin and structure o£ these organelles. The general features of granule development, consisting of the aggregation of subunits into fibers and the cross-linking of these fibers to form a sheet-like matrix on which melanin ...

Aerobic granular sludge (AGS) is based on self-granulated flocs forming mobile biofilms with a gel-like consistence. Bacterial and structural dynamics from flocs to granules were followed in anaerobic-aerobic sequencing batch reactors (SBR) fed with synthetic wastewater, namely a bubble column (BC-SBR) operated under wash-out conditions for fast granulation, and two stirred-tank enrichments of ...

Background: Due to the known disadvantages of autologous bone grafting, tissue engineering approaches have become an attractive method for ridge augmentation in dentistry. To the best of our knowledge, this is the first study conducted to evaluate the potential therapeutic capacity of PRP-assisted hADSCs seeded on HA/TCP granules on regenerative healing response of canine alveolar surgical bone...

Fifteen days after thyroidectomy, the pituitary acidophils of rats were definitely deprived of their large granules , about 350mƒÊ in diameter each, and in due succession, they displayed the changes characterized by the features of "thyroidectomy cells"(TXcells). When these rats were given a single or three injections of thyroxine, 50mg/rat, most of TX-cells might revert to their original acido...