Cell suspension cultures of two chickpea (Cicer arietinum L.) cultivars, resistant and susceptible towards the chickpea pathogen Ascochyta rabiei, were compared with regard to elicitor-induced changes in phytoalexin and isoflavone accumulation. The elicitor was isolated from fermentergrown mycelium of A. rabiei and it mainly consisted of glucose, mannose and N-acetylgalactosamin. Time course an...

The inner carbohydrate was resistant to enzymatic attack and consisted of about one-third of the total mannose and one-half of the total iV-acetylglucosamine residues. It was shown that the carbohydrate chain was not attached to serine or threonine and that it is probably linked to asparagine. The cx and p anomeric forms of galactose were present in about a 1: 1 ratio. Glycopeptides containing ...

Mannose-terminated glucocerebrosidase (alglucerase; Ceredase) was designed for enzyme replacement therapy in Gaucher disease to take advantage of mannose receptor-mediated endocytosis by macrophages. To provide a rational basis for designing enzyme replacement therapy protocols, we examined the in vitro binding, uptake, and degradation of alglucerase by murine and human macrophages. Both were f...

The metabolism of mannose by human erythrocytes has been investigated. Phosphorylation of mannose is achieved by an enzyme with electrophoretic mobility on starch gel indistinguishable from the glucose-phosphorylating enzyme. Mannose phosphorylation is competitively inhibited by glucose; glucose phosphorylation is competitively inhibited by mannose. The K(i) values of inhibition are similar to ...

Worldwide, urinary tract infections (UTIs) are reported in 150-250 million people per year. Annual direct and indirect costs of UTIs for overall patients estimated to be from $2.4 billion $3.5 Uropathogenic Escherichia coli is the most common cause UTIs. These bacteria have ability colonize urothelium, persist epithelial cells, form quiescent biofilms. This bacterial reservoir may provide a sou...

• Get fluorescent glyco-BODIPYs by the combination of a Heck-like cross coupling and Knoevenagel condensation. The glycosidase stable BODIPY-mannose acts as efficient DC-SIGN targeting probes in vitro. BODIPY-mannose: high performant tool fluorescence STED microscopy. functional modular glyco-probe can be further functionalized with bioactive compounds. Significant perspectives on tracking lect...

Sugar-specific recognition is now well established as an important determinant of cell-cell interactions and host defence mechanisms. Macrophages, in particular, are known to express a variety of lectin-like proteins that are specific for oligosaccharides terminating in mannose, fucose, galactose and sialic acid. This review focuses on mannose recognition systems. Terminal mannose is rarely fou...

The mannose receptor is a single polypeptide transmembrane glycoprotein expressed on the surface of macrophages that binds and internalizes soluble and particulate ligands. Physiological ligands for this receptor are pathogens, such as mycobacteria, and extracellular acid hydrolases and peroxidases. Expression of the mannose receptor is tightly linked to the functional state of the macrophage: ...

In fibroblasts from five patients with carbohydrate-deficient glycoprotein syndrome type 1, the incorporation of [2-3H] mannose into mannose phosphates, GDP-mannose, GDP-fucose, dolichol-P-mannose, lipid-linked oligosaccharides, and glycoprotein fraction was determined. We observed a 3- to 5-fold reduction of incorporation of radioactivity into mannose 1-phosphate, GDP-mannose, GDP-fucose, doli...

This study examines the potential of poly(lactic-co-glycolic acid) (PLGA) nanoparticles functionalized with poly(zwitterion)-mannose brushes to target macrophages. Uptake studies RAW 264.7 macrophages indicate that multiple mannose-binding sites in grafted facilitate interaction mannose receptor macrophages, resulting approximately four times higher cellular uptake than monolayer coatings. To t...