Manganese peroxidase (MnP) and lignin peroxidase (LiP) are two major peroxidases involved in lignin biodegradation. The cDNA mnp1 encoding a kind of MnPs, and cDNA clg5 encoding a kind of LiPs were fused to one cDNA mnp1clg5 (rmc15) by over-lap PCR technology in this research. Then the recombinant cDNA rmc15 was cloned into a vector pTrcHisB to construct its efficient expression plasmid pTHmc15...
