Burkholderia pseudomallei is not represented in the current version of Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system. A total of 66 isolates of B. pseudomallei, including 30 clinical isolates collected from National Taiwan University Hospital (NTUH, n = 27) and Peking Union Medical College Hospital (PUMCH, n = 3), and 36 isola...

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been suggested as a reliable method for bacterial identification from cultures. Direct analysis of clinical samples might increase the usefulness of this method, shortening the time for microorganism identification. We compared conventional methods for the diagnosis of urinary tract infections (UTIs)...

Charles River AccuPRO-ID®, the first practical proteotypic identification method, utilizes matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. For industries that are required to identify microorganisms on a regular basis, the ideal technology is one that is accurate, reproducible, fast and inexpensive. MALDI-TOF technology is less dependent on growth condi...

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a useful techniques that allows to derive the spatial distribution of numerous molecules ionized on a tissue section. Selection of the suitable matrix is the key to ionize molecules of interest in MALDI. Each matrix has its own characteristics such as being proper for ionizing molecules in either positive or ne...

1.1 Motivation Since its inception in the mid-1980’s, matrix assisted laser desorption/ionization (MALDI) has revolutionized analytical and biological chemistry by making possible the detection of large, intact biological molecules in the gas phase. This technique also motivated improvements in mass spectrometry, resulting in significant improvements in resolution and mass accuracy for biologic...

BACKGROUND Early identification of pathogens from blood cultures using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry may optimize the choice of empirical antibiotic therapy in the setting of bloodstream infections. We aimed to assess the impact of this new technology on the use of antibiotic treatment in patients with gram-negative bacteremia. METHOD...

Sodium dodecyl sulfate (SDS) is a strong surfactant that is widely used in protein sample preparation. While protein and peptide samples containing up to approximately 1% SDS can be analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) using a two-layer matrix/sample deposition method, the presence of SDS in a protein sample generally degrades mass resolution and ...

BACKGROUND Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry accurately identifies both selected bacteria and bacteria in select clinical situations. It has not been evaluated for routine use in the clinic. METHODS We prospectively analyzed routine MALDI-TOF mass spectrometry identification in parallel with conventional phenotypic identification of bacte...

Bacillus are aerobic spore-forming bacteria that are known to lead to specific diseases, such as anthrax and food poisoning. This study focuses on the characterization of these bacteria by the detection of lipids extracted from 33 well-characterized strains from the Bacillus and Brevibacillus genera, with the aim to discriminate between the different species. For the purpose of analysing the li...

The genus Pseudoalteromonas constitutes an ecologically significant group of marine Gammaproteobacteria with potential biotechnological value as producers of bioactive compounds and of enzymes. Understanding their roles in the environment and bioprospecting for novel products depend on efficient ways of identifying environmental isolates. Matrix Assisted Laser Desorption/Ionization-Time of Flig...