INTRODUCTION Clinical failure of clindamycin therapy has been reported due to multiple mechanisms that confer resistance to macrolide, lincosamide and streptogramin antibiotics. This study was undertaken to detect the presence of inducible clindamycin resistance among clinical isolates of staphylococci. MATERIALS AND METHODS The detection of inducible clindamycin resistance was performed by D...

This is the largest Libyan study to date to investigate the prevalence and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) among health care workers in Tripoli, Libya. A total of 569 doctors and nurses from 4 main hospitals were screened for MRSA with specimens collected from the anterior nares. Isolates from 109 of the 569 subjects (19%) were confirmed as MRS...

Methicillin-resistant Staphylococcus aureus (MRSA) infections have become common among both hospitalized and nonhospitalized patients. Optimal outpatient therapy for MRSA infections has yet to be determined, but this matter is complicated by the possibility of inducible macrolide-lincosamide-streptogramin B resistance (MLSBi). We studied the prevalence of MLSBi in community- and hospital-associ...

The antibacterial activity of NVP-PDF386 (VRC4887), a novel peptide deformylase (PDF) inhibitor, was tested against over 1000 recent clinical isolates collected during 2001 and 2002. The MIC(50/90) (mg/L) results for NVP-PDF386 (VRC4887) were: Staphylococcus aureus (SA) 0.5/1, coagulase-negative staphylococci (CoNS) 0.5/1, Streptococcus pneumoniae 0.25/0.5, other streptococci 0.25/0.5, enteroco...

Induction of resistance to macrolide-, lincosamide-, and streptogramin B-type antibiotics in Staphylococcus aureus was studied by monitoring the appearance of erythromycin A (EM)-resistant [14C]leucine incorporation. Examination of the induction process revealed saturation kinetics and a time course much like that reported for penicillinase in gram-positive bacteria. Induction kinetics in expon...

The behaviors of the Macrolide-Lincosamide-Streptogramin (MLS) resistance genes were investigated in an anaerobic-aerobic pilot-scale system treating spiramycin (SPM) production wastewater. After screening fifteen typical MLS resistance genes with different mechanisms using conventional PCR, eight detected genes were determined by quantitative PCR, together with three mobile elements. Aerobic s...

A high prevalence of macrolide resistant isolates has been found among staphylococci isolated from animals (4). The erm(C) gene is the most common gene encoding macrolide resistance in staphylococci (8). It is well known that expression of the erm(C) gene is normally regulated by formation of hairpin structures upstream for the erm(C) gene (5,9) rendering the start codon of erm(C) gene non-acce...

Increasing frequency of methicillin resistant Staphylococcus aureus (MRSA) infections and changing patterns in antimicrobial resistance have led to renewed interest in the use of macrolide-lincosamide-streptogramin (MLS) antibiotics to treat such infections. Inducible macrolides, lincosamides, type B streptogramins (MLS(Bi)) resistance has to be identified to avoid clinical failure of clindamyc...