نتایج جستجو برای: m pcr
تعداد نتایج: 703897 فیلتر نتایج به سال:
macrophage colony stimulating factor (m-csf) has previously been shown to affect the differentiation of cells of the mono-nuclear phagocytic line. more recent studies indicate that m-csf may have a role in pregnancy. in the present study, the expression of m-csf in the human placenta was demonstrated. placental mrna was isolated and used as template for synthesis of complementary dna (cdna). th...
ichthyozoonotic mycobacterium spp. poses health risks both to fish and humans. in this study, the presence of ichthyozoonotic mycobacterium spp. was investigated in red mullet (mullus barbatus barbatus) and surmullet (mullus surmuletus), widely caught species in the mediterranean and the aegean sea. a total of 208 fish samples, provided from fishermen of mersin province (turkey) were studied. u...
AIMS To develop an immunomagnetic capture (IMC) to detect viable Mycoplasma agalactiae in routine ovine milk samples. METHODS AND RESULTS Polyclonal antibodies against two M. agalactiae membrane surface proteins (P80 and P55) were covalently conjugated to magnetic beads (MBs) to form MB-Ab80 and MB-Ab55. Mycoplasma agalactiae cells were captured by a specific antigen-antibody reaction and mag...
Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.
Background and Aims: Newcastle disease virus (NDV) is an avian paramyxovirus (A-PMV 1) and one of the major pathogens in poultries. Vaccination is intended to control the disease, nevertheless this virus is a growing threat to the poultry industry. So, early detection of the virus can prevent the spread of illness and avoid huge economic losses. Towards this goal, in this research, we develop...
خلاصهاشریشیاکلی تولیدکننده توکسین مشابه شیگا (stec) (وروتوکسیژنیک) در سال های اخیر به عنوان مهمترین گروه از پاتوژن های مواد غذایی مطرح شده است. اصلی ترین جایگاه این باکتری ها در طبیعت، روده گاو است که از این طریق سبب آلوده شدن مواد غذایی با منشاء گاو می شود. هدف از انجام این مطالعه جداسازی و تشخیص فراوانی سویه های stec با روش کشت اختصاصی و multiplex pcr در مدفوع گاوهای شهر کرمان بود.در این مطال...
background and aims: newcastle disease virus (ndv) is an avian paramyxovirus (a-pmv 1) and one of the major pathogens in poultries. vaccination is intended to control the disease, nevertheless this virus is a growing threat to the poultry industry. so, early detection of the virus can prevent the spread of illness and avoid huge economic losses. towards this goal, in this research, we developed...
Mycobacterium abscessus (M. abscessus sensu lato, or the M. abscessus group) comprises three closely related taxa whose taxonomic statuses are under revision, i.e., M. abscessus sensu stricto, Mycobacterium bolletii, and Mycobacterium massiliense. We describe here a simple, robust, and cost-effective PCR-based method for distinguishing among M. abscessus, M. massiliense, and M. bolletii. Based ...
Mastitis is one of the main diseases that affects dairy cattle. It characterized by a multifactorial disease with multiple etiologies including microorganisms such as bacteria, viruses, fungi, yeasts, and algae. caused Mycoplasma spp. results in contagious nature infection has been studied much Brazil. The objectives this research were (1) to investigate clinical mastitis milk samples, conventi...
background : this study was to evaluate the prevalence of ctx-mand tem type esbls-producing k. pneumoniae and determination of mdr, xdr, and pdr phenotypes of these isolates as well as find out the genetic relationship and molecular typing of these isolates using phenotypic and genotypic methods. methods : non-repetitive 96 k. pneumonia isolates were isolated from hospitalized patients in a...
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