نتایج جستجو برای: m pcr

تعداد نتایج: 703897  

Journal: :مجله دانشکده پزشکی دانشگاه علوم پزشکی تهران 0
رخند آروان r arvan الهه الهی e elahy

macrophage colony stimulating factor (m-csf) has previously been shown to affect the differentiation of cells of the mono-nuclear phagocytic line. more recent studies indicate that m-csf may have a role in pregnancy. in the present study, the expression of m-csf in the human placenta was demonstrated. placental mrna was isolated and used as template for synthesis of complementary dna (cdna). th...

Journal: :iranian journal of veterinary research 2015
p. sevim s. ozer f. rad

ichthyozoonotic mycobacterium spp. poses health risks both to fish and humans. in this study, the presence of ichthyozoonotic mycobacterium spp. was investigated in red mullet (mullus barbatus barbatus) and surmullet (mullus surmuletus), widely caught species in the mediterranean and the aegean sea. a total of 208 fish samples, provided from fishermen of mersin province (turkey) were studied. u...

Journal: :Journal of applied microbiology 2014
G Sanna V Lecca A Foddai S Tola

AIMS To develop an immunomagnetic capture (IMC) to detect viable Mycoplasma agalactiae in routine ovine milk samples. METHODS AND RESULTS Polyclonal antibodies against two M. agalactiae membrane surface proteins (P80 and P55) were covalently conjugated to magnetic beads (MBs) to form MB-Ab80 and MB-Ab55. Mycoplasma agalactiae cells were captured by a specific antigen-antibody reaction and mag...

2009
Eduardo Eustáquio de Souza Figueiredo Flávia Galindo Silvestre Wilma Neres Campos Leone Vinícius Furlanetto Luciana Medeiros Walter Lilenbaum Leila Sousa Fonseca Joab Trajano Silva Vânia Margaret Flosi Paschoalin

Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.

B Zahraei Zahraei , E Zafari , M Najarasl , M Sharti , M Tat , MS Hashemzadeh , R Dorostkar , S Zahiriyeganeh ,

Background and Aims: Newcastle disease virus (NDV) is an avian paramyxovirus (A-PMV 1) and one of the major pathogens in poultries. Vaccination is intended to control the disease, nevertheless this virus is a growing threat to the poultry industry. So, early detection of the virus can prevent the spread of illness and avoid huge economic losses. Towards this goal, in this research, we develop...

ژورنال: :فصلنامه دامپزشکی و آزمایشگاه 0
محمد خلیلی khalili, m. research center of animal genetic and transgenic animals, shahid bahonar university of kerman, kerman, iran . research center of animal genetic and transgenic animals, shahid bahonar university of kerman, kerman, iran .هسته تحقیقاتی ژنتیک حیوانی و حیوانات ترانسژنیک دانشگاه شهید باهنرکرمان، کرمان، ایران. شهلا منصوری mansouri, sh. ملیحه افضلی afzali, m محمد رضا محمد آبادی mohammad-abadi, m.r.

خلاصهاشریشیاکلی تولیدکننده توکسین مشابه شیگا (stec) (وروتوکسیژنیک) در سال های اخیر به عنوان مهمترین گروه از پاتوژن های مواد غذایی مطرح شده است. اصلی ترین جایگاه این باکتری ها در طبیعت، روده گاو است که از این طریق سبب آلوده شدن مواد غذایی با منشاء گاو می شود. هدف از انجام این مطالعه جداسازی و تشخیص فراوانی سویه های stec با روش کشت اختصاصی و multiplex pcr در مدفوع گاوهای شهر کرمان بود.در این مطال...

Journal: :iranian journal of virology 0
b zahraei zahraei applied virology research center, baqiyatallah university of medical sciences, tehran, iran ms hashemzadeh applied virology research center, baqiyatallah university of medical sciences, tehran, iran ms hashemzadeh applied virology research center, baqiyatallah university of medical sciences, tehran, iran m najarasl applied virology research center, baqiyatallah university of medical sciences, tehran, iran s zahiriyeganeh applied virology research center, baqiyatallah university of medical sciences, tehran, iran e zafari applied virology research center, baqiyatallah university of medical sciences, tehran, iran

background and aims: newcastle disease virus (ndv) is an avian paramyxovirus (a-pmv 1) and one of the major pathogens in poultries. vaccination is intended to control the disease, nevertheless this virus is a growing threat to the poultry industry. so, early detection of the virus can prevent the spread of illness and avoid huge economic losses. towards this goal, in this research, we developed...

Journal: :Journal of clinical microbiology 2013
Shamira J Shallom Paul J Gardina Timothy G Myers Yinong Sebastian Patricia Conville Leslie B Calhoun Hervé Tettelin Kenneth N Olivier Gulbu Uzel Elizabeth P Sampaio Steven M Holland Adrian M Zelazny

Mycobacterium abscessus (M. abscessus sensu lato, or the M. abscessus group) comprises three closely related taxa whose taxonomic statuses are under revision, i.e., M. abscessus sensu stricto, Mycobacterium bolletii, and Mycobacterium massiliense. We describe here a simple, robust, and cost-effective PCR-based method for distinguishing among M. abscessus, M. massiliense, and M. bolletii. Based ...

Journal: :Ruminants 2023

Mastitis is one of the main diseases that affects dairy cattle. It characterized by a multifactorial disease with multiple etiologies including microorganisms such as bacteria, viruses, fungi, yeasts, and algae. caused Mycoplasma spp. results in contagious nature infection has been studied much Brazil. The objectives this research were (1) to investigate clinical mastitis milk samples, conventi...

Journal: :journal of medical bacteriology 0
zahra tahanasab department of microbiology, school of medicine, isfahan university of medical sciences, isfahan, iran. sina mobasherizadeh department of microbiology, school of medicine, isfahan university of medical sciences, isfahan, iran. mehdi moghadampour department of microbiology, school of medicine, isfahan university of medical sciences, isfahan, iran. aliakbar rezaei department of microbiology, school of medicine, isfahan university of medical sciences, isfahan, iran. nafiseh maleki department of microbiology, school of medicine, isfahan university of medical sciences, isfahan, iran. jamshid faghri department of microbiology, school of medicine, isfahan university of medical sciences, isfahan, iran.

background :   this study was to evaluate the prevalence of ctx-mand tem type esbls-producing k. pneumoniae and determination of mdr, xdr, and pdr phenotypes of these isolates as well as find out the genetic relationship and molecular typing of these isolates using phenotypic and genotypic methods. methods :   non-repetitive 96 k. pneumonia isolates were isolated from hospitalized patients in a...

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