Cells must regulate the abundance and activity of numerous nutrient transporters in different organelle membranes to achieve nutrient homeostasis. As the recycling center and major storage organelle, lysosomes are essential for maintaining nutrient homeostasis. However, very little is known about mechanisms that govern the regulation of its membrane proteins. In this study, we demonstrated that...

Dyes for measuring lysosome pH (pHlys) cannot be propagated in cells longitudinal or vivo studies. We report a new genetically encoded ratiometric pHlys biosensor, pHLARE (pH Lysosomal Activity REporter), which we use to show decreased with inhibiting mTORC1 (mammalian target of rapamycin complex 1) activity and cancer different tissues genetic signatures.

Abstract Cathepsin A (CTSA) is a lysosomal protease that regulates galactoside metabolism. The previous study has shown CTSA abnormally expressed in various types of cancer. However, rarely the addressed role hepatocellular carcinoma (HCC) and its prognostic value. To clinical value potential function HCC, datasets from Cancer Genome Atlas (TCGA) database 136 HCC patient cohort were analyzed. e...