Phenylalanine ammonia-lyase (PAL) is the first enzyme in phenylpropanoid pathway and plays a vital role adoption, growth, development plants but wheat its characterization still not very clear. Here, we report genome-wide identification of TaPAL genes analysis their transcriptional expression, duplication, phylogeny wheat. A total 37 that cluster into three subfamilies have been identified base...

The enzyme CYP17 primarily regulates androgen production by mediating four reactions: conversion of pregnenolone and progesterone to 17-hydroxypregnenolone and 17-hydroxyprogesterone, respectively (17alpha-hydroxylase activity), followed by conversion of the 17-hydroxylated steroids to dehydroepiandrosterone and androstenedione, respectively (17,20-lyase activity). Most mammalian CYP17 isoforms...

A cDNA clone for phenylalanine ammonia-lyase (PAL) induced in wounded sweet potato (Ipomoea batatas Lam.) root was obtained by immunoscreening a cDNA library. The protein produced in Escherichia coli cells containing the plasmid pPAL02 was indistinguishable from sweet potato PAL as judged by Ouchterlony double diffusion assays. The M(r) of its subunit was 77,000. The cells converted [(14)C]-l-p...

Abiotic stress, like heat treatment and wounding, applied to pineapple by-products induce the accumulation of new compounds add value. In this work effect individual or combined application wounding stresses on total phenolic content, antioxidant activity through complementary methods (DPPH, FRAP, ABTS) enzymatic (bromelain, phenylalanine ammonia lyase (PAL) polyphenol oxidase) were evaluated. ...

The influence of vegetal extracts derived from red grape, blueberry fruits and hawthorn leaves on Zea mays L. plant growth and the activity of phenylalanine ammonia-lyase (PAL), a key enzyme of the phenylpropanoid pathway, was investigated in laboratory experiments. The extracts were characterized using FT-IR and Raman spectroscopies in order to obtain a pattern of the main functional groups. I...

Trichosporon cutaneum phenylalanine ammonia lyase was selected as a model to investigate the dual substrate activity of this family of enzymes. Sequencing of the PAL gene identified an extensive intron region at the N-terminus. Five amino acid residues differing from a prior report were identified. Highest Phe : Tyr activities (1.6  ± 0.3 : 0.4 ± 0.1  μ mol/h g wet weight) were induced by Tyr. ...

Alginate lyase mainly produces active alginate oligosaccharides (AOS) by degrading via β-elimination process. In this study, the Pseudoalteromonas sp. Alg6B lyase-encoding gene alg6B-7 from polysaccharide (PL)-7 family was successfully cloned, sequenced, expressed in Escherichia coli. Based on rational design and amino acid sequence alignment of various sources, four positive mutants were obtai...

In a basic investigation, the antioxidative responses of two maize inbreds, A-180 and A-619 were studied. The results revealed that during drought period the total antioxidant capacity is quickly increased in leaf and root tissues of both plants, while it is slowly reverted back to the normal level during recovery. The activity of Pheylalanine ammonia lyase (PAL), a key enzyme involved in the b...