The pneumolysin toxoid, Δ6 PLY, is a prototype pneumococcal protein vaccine candidate. However, its potentially detrimental residual pro-inflammatory interactions with human neutrophils are unknown. In the current study the effects of the toxoid (8-1000 ng/ml) have been compared with those of wild-type pneumolysin (WT/PLY, 8 ng/ml) on neutrophil cytosolic Ca(2+) fluxes, generation of leukotrien...

Hookworm infections and tuberculosis (TB) are coendemic in many parts of the world. It has been suggested that infection with helminth parasites could suppress the predominant Th1 (IFN-γ-mediated) response needed to control Mycobacterium tuberculosis infection and enhance susceptibility to infection and/or disease. To determine the role of coincident hookworm infection on responses at steady-st...

The accumulation of eosinophils in inflammatory foci is a hallmark characteristic of Th2 inflammation. Nevertheless, the expression of inhibitory receptors such as paired immunoglobulin-like receptor B (PIR-B) and their function regulating eosinophil accumulation have received limited attention. We now report that Pirb was up-regulated in an eosinophil-dependent manner in the lungs of allergen-...

Japanese encephalitis virus (JEV) is an enveloped icosahedral capsid with a prime neutralizing epitope present in E protein domain III (EDIII). dimers are rearranged into five-fold symmetry of icosahedrons. Cholera toxin B (CTB) and heat-labile enterotoxin (LTB) AB5-type was used as the structural scaffold for emulating pentameric axis EDIII. We produced homo-pentameric EDIII through genetic fu...

We provide a formula for estimating the redshift and its secular change (redshift drift) in Lema\^{\i}tre-Tolman-Bondi (LTB) spherically symmetric universes. compute scaling of drift LTB models that predict Hubble diagrams indistinguishable from those standard cosmological model, flat lambda cold dark matter ($\mathrm{\ensuremath{\Lambda}}\mathrm{CDM}$) model. show these degenerate is typically...

In this study, nineteen 3-ply laminated glass beams of 1.0 m span with different and interlayer types were tested to failure. Annealed generally failed in shear facture, whereas toughened displayed lateral-torsional buckling (LTB) response, a bow-shaped out-of-plane deflected shape significant bending. The experimental failure loads had scatter for nominally similar beams, highlighting the vari...

the performance of selective media ltb, (leauryl tryptose broth), mmgm (minerals modified glutamate medium), bgbb (brilliant green bile broth), for the detection of e.coli in water in water, were compared and the following result were obtained: the mmgm found to be unsuitable as confirmatory medium for the detection of e.coli at 44 c, due to the occurrence of an unacceptable number of the false...

Leukotriene B (LTB), a potent lipid chemotactic factor for neutrophils, is 5S,12R-dihydroxy-6,14-cis,8,10-trans-eicosatetraenoic acid (Fig 1), based upon direct comparison of natural LTB with synthetic 5S,12R-dihydroxy-6,8,10,14-eicosatetraenoic acid (5,12-di-HETE) stereoisomers in three biological assays. Of the six synthetic stereoisomers evaluated, only the 5S,12R,6,14-cis,8,10-trans compoun...

THE acyl carrier coenzyme A (CoA) is involved in fatty acid metabolism. The carnitine/CoA ratio is of particular importance in regulating the transport of long-chain fatty acids into mitochondria for oxidation. Also CoA has a role in the formation and breakdown of products from both the cyclooxygenase and lipoxygenase pathways of the precursor arachidonic acid. In the present study the effect o...

Original Equipment Manufacturers (OEM’s) of advanced capital goods often offer service contracts for system support to their customers, for which spare parts are needed. Due to technological changes, suppliers of spare parts may stop production at some point in time. As a reaction to that decision, an OEM may place a so-called Last Time Buy (LTB) order to cover demand for spare parts during the...