High resolution cryoEM of mammalian mitoribosomes revealed the unexpected presence of mitochondrially encoded tRNA as a structural component of mitochondrial large ribosomal subunit (mt-LSU). Our previously published data identified that only mitochondrial (mt-) tRNAPhe and mt-tRNAVal can be incorporated into mammalian mt-LSU and within an organism there is no evidence of tissue specific variat...

Computational science, interdisciplinary research and the use of information technology are transforming education, research, and institutional culture in academic institutions. In the last decade, Louisiana has targeted significant resources to these areas, one outcome of which has been the establishment of the Center for Computation & Technology (CCT) at Louisiana State University (LSU). This...

Defects of the translation apparatus in human mitochondria are known to cause disease, yet details of how protein synthesis is regulated in this organelle remain to be unveiled. Here, we characterize a novel human protein, C7orf30 that contributes critically to mitochondrial translation and specifically associates with the large subunit of the mitochondrial ribosome (mt-LSU). Inactivation of C7...

Cress (Lepidium sativum) seeds were germinated in darkness. Seedlings were investigated for soluble proteins by SDS-PAGE. Two proteins were identified by microsequencing: the small subunit of ribulosebisphosphate carboxylase (SSU) and the alpha subunit of the storage protein cruciferin. Net synthesis of small and large subunits of ribulosebisphosphate carboxylase (SSU and LSU) was investigated ...

ii ACKNOWLEDGEMENTS I would like to take this opportunity to thank my Mother who has been most supportive and has been my source of inspiration whenever I needed direction. I would like to thank my advisor, Dr. Iyengar for his invaluable guidance throughout my Ph.D. studies at LSU. I learnt the importance of honesty, integrity, creativity, critical-thinking, and hard work as a researcher throug...

Flukes belonging to Sphaeridiotrema are important parasites of waterfowl, and 2 morphologically similar species Sphaeridiotrema globulus and Sphaeridiotrema pseudoglobulus, have been implicated in waterfowl mortality in North America. Cytochrome oxidase I (barcode region) and partial LSU-rDNA sequences from specimens of S. globulus and S. pseudoglobulus, obtained from naturally and experimental...

Leishmania is a single-cell eukaryotic parasite of the Trypanosomatidae family, whose members cause an array of tropical diseases. The often fatal outcome of infections, lack of effective vaccines, limited selection of therapeutic drugs, and emerging resistant strains, underline the need to develop strategies to combat these pathogens. The Trypanosomatid ribosome has recently been highlighted a...

Fresh collections, type studies and molecular phylogenetic analyses of a multigene matrix of partial nuSSU-ITS-LSU rDNA, rpb2, tef1 and tub2 sequences were used to evaluate the boundaries of Cucurbitaria in a strict sense and of several related genera of the Cucurbitariaceae. Two species are recognised in Cucurbitaria and 19 in Neocucurbitaria. The monotypic genera Astragalicola, Cucitella, Par...

Prevalensi prolaps uteri tergolong tinggi dan meningkat sejalan dengan paritas. Angka tersebut akanterus karena usia harapan hidup perempuan juga meningkat. Hal berdampak padakualitas perempuan. Faktor risiko klinis yang saling terkait proses perkembangan adalahkehamilan, persalinan, berat lahir bayi, obesitas, menopause, kebiasaan mengangkat benda berat, batuklama, konstipasi. Kerusakan penyok...