1. Perfusion of the isolated rat liver was accompanied by a rising level of transcobalamin I1 in the perfusate. There was no change in the concentration of transcobalamin I over the 5 h perfusion period. 2. When [G-14C]leucine was added to the perfusate a 14C-labelled protein appeared in the same position as transcobalamin I1 on column chromatography. 3. It was concluded that transcobalamin I1 ...

Ischaemia-reperfusion injury (IRI) is the leading cause of injury seen in the liver following transplantation. IRI also causes injury following liver surgery and haemodynamic shock. The first cells within the liver to be injured by IRI are the liver sinusoidal endothelial cells (LSEC). Recent evidence suggests that LSEC co-ordinate and regulates the livers response to a variety of injuries. It ...

In previous studies, we have shown that a relatively large amount of low density lipoproteins is released into the perfusate during isolated pig liver perfusion. The present studies were done to determine the source of these lipoproteins. Breakdown of the very low density lipoproteins to low density lipoproteins by the perfusion apparatus or by hepatic catabolism was excluded by adding 125I ver...

Liver segmentation in MR Image is the first step of our automated liver perfusion analysis project. Traditional Level Set methods and active contours were often used to segment the liver, but the results were not always promising due to noise and the low gradient response on the liver boundary. In this paper we propose a novel level set based variational approach that incorporates shape prior k...

sterone or [16B-”H]betamethasone was given at a dose of 0.23pmol/kg body wt. by intraperitoneal or intravenous route, and bile samples were collected at 30min intervals. In some experiments the animals were pretreated with daily doses (100mg/kg body wt., intraperitoneally) of phenobarbital for 3 consecutive days. In the isolated-liver perfusion experiments tissue-culture medium 199 (100ml) cont...

The liver is the main organ responsible for drug metabolism; hepatocyte-based culture models play a fundamental role in understanding physiology. While different types of two-dimensional and three-dimensional have been developed, failure to accurately mimic native liver, including extracellular matrix (ECM) composition, complex structure, rich vascular network as well shortage donors, impede th...

oxygen is an essential part of the most important metabolic pathways in aerobic organisms. oxygen delivery is merely dependent on blood, rendering blood loss a devastating event. traumatic pre-hospital liver bleeding is a major cause of early trauma deaths in human and animals, with no established therapeutic method yet. increasing intra-abdominal pressure (iap) has been shown to reduce liver b...