The iap gene of Listeria species encodes protein p60. The comparison of iap-related genes from different Listeria species indicated common and variable regions within these genes which appeared to be specific for each Listeria species. On the basis of the iap gene sequences, pairs of polymerase chain reaction (PCR) primers which allowed the unambiguous identification of all members of the genus...

The study was carried out to determine the prevalence and the in-vitro pathogenicity of Listeria monocytogenes in sheep and goats reared by nomads of Jammu region of J&K, in addition to biochemical characterization of isolates. A total of 125 samples comprising of sheep and goat faecal samples and their rectal swabs were collected. Isolation of Listeria species involved the enrichment of the sa...

Listeria monocytogenes can cause serious infection and recently, relapse of listeriosis has been reported in leukemia and colorectal cancer, and the patients with Klebsiella pneumoniae are at increased risk of colorectal cancer. Translation initiation codon recognition is basically mediated by Shine-Dalgarno (SD) and the anti-SD sequences at the small ribosomal RNA (ssu rRNA). In this research,...

Accurate and rapid detection of Listeria is of significant interest to the food industry. The 3MTM PetrifilmTM Environmental Listeria Plate has been developed in order to provide a Listeria result from environmental samples within 27 to 31 hours. This study compared the Petrifilm Plate method with ISO 11290-2 using PALCAM agar for detection and enumeration of Listeria. Studies of 88 strains dem...

We have studied IL-12p35-deficient (IL-12p35(-/-)) mice to evaluate the role of IL-12 in resistance against Listeria monocytogenes. In the absence of bioactive IL-12p75, mutant mice acquired higher bacterial organ burden than wild-type mice and died during the first week following infection with normally sublethal doses of Listeria. Moreover, blood IFN-gamma levels were strikingly reduced in mu...

Testing for Listeria spp. was performed in milk samples collected from Syrian provinces, from August 2005 to December 2008. A total of 766 milk samples were collected from randomly selected dairy farms. Using conventional bacteriologic methods, 84 samples (10.96%) were positive for Listeria spp. The isolated Listeria spp. strains were catalase positive, oxidase negative, did not produce H2S and...

........................................................................................................................... II ACKNOLEDGEMENTS ..................................................................................................... III LIST OF TABLES ................................................................................................................ VI LIST OF FIGURES ....

Listeria monocytogenes was successfully isolated from experimentally infected mice by placing homogenized tissues in phosphate-buffered saline (PBS), trypsin, peptone, or pepsin followed by incubation at 37 C for 24 hr. A larger number of Listeria isolates were recovered from the trypsin or PBS splenic homogenate suspensions incubated at 37 C for 2 hr than from the other diluent suspensions. Ho...

AIMS This study investigated the performance of a new chromogenic plating medium for the detection of Listeria monocytogenes from naturally contaminated samples obtained from marine environments in Morocco in comparison with the conventional plating media PALCAM and Oxford. METHODS A total of 479 marine samples (sea water, sediment and mussels) were collected from 16 littoral sites in the reg...