Membrane assisted liquid extraction(MALE) technologies are gaining an important role as an extraction /separation technique for actinides and are being deployed as a  promising tool for remediation of nuclear waste generated in the reprocessing plant and other radioactive wastes containing a trace level of radionuclides. The present contribution outlines the classification of membrane assisted ...

The failure of most non-ionic detergents to release patches of DRM (detergent-resistant membrane) at 37 degrees C undermines the claim that DRMs consist of lipid nanodomains that exist in an L(o) (liquid ordered) phase on the living cell surface. In the present study, we have shown that inclusion of cations (Mg(2+), K(+)) to mimic the intracellular environment stabilizes membranes during solubi...

A comprehensive study pertaining to the emulsion liquid membrane (ELM) extraction process to enrich dilute aqueous solutions of silver salt is presented. The study has highlighted the importance and influence of membrane composition for maximizing the extraction of Ag+ ions. The liquid membrane was made up of Cyanex-302 as an extractant and the industrial solvent mainly consists of p...

Involvement of sterols in membrane structural properties has been extensively studied in model systems but rarely assessed in natural membranes and never investigated for the plant plasma membrane (PM). Here, we address the question of the role of phytosterols in the organization of the plant PM. The sterol composition of tobacco BY-2 cell PM was determined by gas chromatography. The cyclic oli...

The outer membrane-spanning (Oms) proteins of Borrelia burgdorferi have been visualized by freeze-fracture analysis but, until recently, not further characterized. We developed a method for the isolation of B. burgdorferi outer membrane vesicles and described porin activities with single-channel conductances of 0.6 and 12.6 nS in 1 M KCI. By using both nondenaturing isoelectric focusing gel ele...

Overexpression of Pseudomonas aeruginosa outer membrane protein OprH led to an 8- to 32-fold increase in susceptibility to chloramphenicol and the quinolones nalidixic acid, norfloxacin, ciprofloxacin, and fleroxacin in comparison with the susceptibility of the wild-type strain H103 grown on Mg(2+)-sufficient medium. This was true regardless of whether OprH overexpression was induced by growth ...

The enzyme complex sucrase l isomaltase is an intrinsic dimeric glycoprotein with a molecular weight of approximately 220,000. It is anchored in the intestinal brush border membrane by a hydrophobic segment amounting to less than 10% of the total protein mass which is located near the NHz-terminal end of the isomaltase subunit. This conclusion is based on studies of (i) the mechanism of solubil...