Assessment of circulating endotoxin during the perioperative period, which is only demonstrated by the Limulus amebocyte lysate (LAL) test, may be modulated by several endotoxin-binding proteins. Endotoxin-neutralizing capacity (ENC) and the plasma levels of soluble CD14 (sCD14), lipopolysaccharide-binding protein, and bactericidal/permeability-increasing protein (BPI) were determined in 40 pat...

To study the mechanisms of protection against endotoxin challenge offered by antisera to smooth and rough gram-negative organisms, we have developed an assay to quantitate endotoxin neutralization based on inhibition of the Limulus amoebocyte lysate test. Dilutions of different bacterial lipopolysaccharides (LPSs) were incubated with hyperimmune rabbit sera against Escherichia coli O113, E. col...

Semisynthetic penicillins in concentrations greater than 1.0% (wt/vol) were found to inhibit the limulus lysate assay for endotoxin.

Prof. M. Fischbach, Unité Nephrologie Dialyse, Transplantation, Pédiatrie 1, Hôpitaux Universitaires de Strasbourg Hautepierre, F-67000 Strasbourg (France) Patient Dear Sir, Microbially contaminated dialysate is a well-known risk in patients undergoing he-modialysis but today seems very infrequent. On the contrary, more attention is to take into consideration for the dialysate pyrogenic substan...

BACKGROUND Microleakage through the interface has been documented in implant systems with titanium (Ti) abutments. There is a current increase in the use of zirconia (Zi) abutments especially in esthetic zone in where higher risk of visible metal color through the peri-implant tissues exists. PURPOSE The aim of the present in vitro study is to evaluate the leakage at the implant fixture-abutm...

INTRODUCTION Periradicular tissue chronic stimulation by endotoxin may cause apical periodontitis. This study investigated the microbial profile and the levels of endotoxin found in primary root canal infection with apical periodontitis, determined their antigenicity against macrophages through the levels of interleukin (IL)-1beta and tumor necrosis factor alpha (TNF-alpha), and evaluated their...

erythropoietin preparations. as measured with the Limulus lvsate test. ’ As noted by Dr. Fumarola. Limulus amebocyte Ivsate can be used to remove endotoxin from erythropoietin without loss of hormone activity.7 However. such preparations may not be suitable for tissue culture work due to the presence of residual amebocyte protein. Finally. even with removal of the endotoxin, the ratio ofactive ...

Secretion of cytokines by monocytes has been implicated in the pathogenesis of dialysis-related morbidity. Cytokine generation is presumed to take place in two steps: induction of mRNA transcription for cytokines by C5a and direct membrane contact, followed by lipopolysaccharide (LPS)-induced translation of mRNA (priming/second signal theory, Kidney Int 37: 85-93, 1990). However, the in vitro c...

erythropoietin preparations. as measured with the Limulus lvsate test. ’ As noted by Dr. Fumarola. Limulus amebocyte Ivsate can be used to remove endotoxin from erythropoietin without loss of hormone activity.7 However. such preparations may not be suitable for tissue culture work due to the presence of residual amebocyte protein. Finally. even with removal of the endotoxin, the ratio ofactive ...