Association or linkage disequilibrium (LD)-based mapping strategies are receiving increased attention for the identification of quantitative trait loci (QTL) in plants as an alternative to more traditional, purely linkage-based approaches. An attractive property of association approaches is that they do not require specially designed crosses between inbred parents, but can be applied to collect...

Late blight, caused by the pathogen Phytophthora infestans, is one of the most devastating diseases of potato. Here, we describe a new single dominant resistance gene, Rpi2, from the Mexican diploid wild species Solanum pinnatisectum that confers high level and broad spectrum resistance to late blight. The Rpi2 locus confers full resistance to complex isolates of P. infestans, for which race sp...

Reliable and sensitive quantification of Phytophthorainfestansin potato plant is of crucial importance in managing the multiple syndromes caused by this pathogen. A Real-Time polymerase chain reaction (PCR) assay was developed for the determination of P.infestans in host tissues. DNA levels of a highly virulent isolate were measured in different potato cultivars with varying degrees of resistan...

We conducted a study of the patterns and dynamics of oxidized fatty acid derivatives (oxylipins) in potato leaves infected with the late-blight pathogen Phytophthora infestans. Two 18-carbon divinyl ether fatty acids, colneleic acid and colnelenic acid, accumulated during disease development. To date, there are no reports that such compounds have been detected in higher plants. The divinyl ethe...

Potato is the third most important food crop worldwide. However, genetic and genomic research of potato has lagged behind other major crops due to the autopolyploidy and highly heterozygous nature associated with the potato genome. Reliable and technically undemanding techniques are not available for functional gene assays in potato. Here we report the development of a transient gene expression...