The lamins form a fibrous network underlying the inner nuclear membrane termed the nuclear lamina. In order to gain insights into the role of lamins in nuclear organization, we have characterized a monoclonal antibody (LA-2H10) raised against recombinant rat lamin A that labels nuclei in a speckled pattern in all cells of unsynchronized populations of HeLa and rat F-111 fibroblast cells, unlike...

A preparative two-dimensional polyacrylamide gel system was used to separate and purify the major Coomassie blue-stained proteins from the isolated rat liver nuclear matrix. Approximately 12 major proteins were consistently found. Of these, 5 proteins represented identified proteins, including nuclear lamins A, B, and C, the nucleolar protein B-23, and residual components of core heterogeneous ...

BACKGROUND Aberrant expression patterns of nuclear lamins have been described in various types of cancer depending on the subtype of cancer, its aggressiveness, proliferative capacity and degree of differentiation. In general, the expression of A-type lamins (lamins A and C) has been correlated with a non-proliferating, differentiated state of cells and tissues. OBJECTIVES To establish and co...

T he nuclear envelope (NE) embraces the genome with a rich tapestry of outer and inner membrane proteins, some of which mechanically link the cytoskeleton to the nucleoskeleton (1). The nucleoskeleton includes the nuclear intermediate filaments, formed by independent networks of Aand B-type lamins. Humans have one gene (LMNA) encoding A-type lamins and two genes encoding B-type lamins (LMNB1 an...

The nuclear envelope (NE) is a highly dynamic structure consisting of two concentric membranes underlaid by the nuclear lamina, a network of intermediate filaments called A/Cand B-type lamins (reviewed by Collas and Courvalin, 2000; Wilson, 2000). The outer nuclear membrane (ONM) is in direct continuity with the endoplasmic reticulum (ER) and shares biochemical and functional properties with th...

INTRODUCTION Although nuclei are often spherical, nuclear shape can change significantly between cell types and during cell differentiation or aging (Dauer and Worman, 2009). The nuclear envelope (NE) is a key determinant of nuclear morphology. The nuclear lamina, a meshwork underlying the inner nuclear membrane in metazoa, is crucial for the structural integrity of nuclei (Stuurman et al., 199...

The nuclear lamina is a meshwork of intermediate-type filament proteins (lamins) that lines the inner nuclear membrane. The lamina is proposed to be an important determinant of nuclear structure, but there has been little direct testing of this idea. To investigate lamina functions, we have characterized a novel lamin B1 mutant lacking the middle approximately 4/5 of its alpha-helical rod domai...

Regulation of gene activity is mediated by alterations in chromatin organization. In addition, chromatin organization may be governed in part by interactions with structural components of the nucleus. The nuclear lamins comprise the lamina and a variety of nucleoplasmic assemblies that together are major structural components of the nucleus. Furthermore, lamins and lamin-associated proteins hav...

Lamins are nuclear intermediate filament proteins and the major building blocks of the nuclear lamina. Besides providing nuclear shape and mechanical stability, lamins are required for chromatin organization, transcription regulation, DNA replication, nuclear assembly, nuclear positioning, and apoptosis. Mutations in human lamins cause many different heritable diseases, affecting various tissue...

Lamins form structural filaments in the nucleus. Mutations in A-type lamins cause muscular dystrophy, cardiomyopathy and other diseases, including progeroid syndromes. To identify new binding partners for lamin A, we carried out a two-hybrid screen with a human skeletal-muscle cDNA library, using the Ig-fold domain of lamin A as bait. The C-terminal region of titin was recovered twice. Previous...