The release of lamellar bodies from rat alveolar type 2 cells has been studied during a 12hr dark/12hr light cycle. A proportion of the type 2 cell population was ultrastructurally abnormal. The cellular changes formed a spectrum raninging from mild to severe, culminating in rupture of the cell and release of its contents into the alveolar air space. The majority of aberrant cells were observed...

OBJECTIVE To describe the use of a single donor corneal tissue in 3 patients with corneal pathologic conditions. METHODS A donor corneal tissue was divided into 3 parts using a microkeratome and a trephine. The anterior lamellar disc was transplanted into a patient with macular corneal dystrophy using the automated lamellar therapeutic keratoplasty technique. The posterior lamellar disc was t...

Corneal transplantation, as full-thickness transplantation, is an old surgical procedure which has been successfully performed for more than 100 years. However, in a last decade significant changes in surgical technique(s) have been implemented. The reason for such changes is the current trend to replace only the diseased part of the recipient cornea, not the whole cornea. Therefore, if the ant...

STUDY DESIGN Basic science study using in vitro tissue testing and imaging to characterize local strains in annulus fibrosus (AF) tissue. OBJECTIVE To characterize mesoscale strain inhomogeneities between lamellar and inter-/translamellar (ITL) matrix compartments during tissue shear loading. SUMMARY OF BACKGROUND DATA The intervertebral disc is characterized by significant heterogeneities ...

We propose a model for the liquid-liquid (L(alpha)-->L(alpha(') )) phase transition observed in osmotic pressure measurements of certain charged lamellae-forming amphiphiles. The model free energy combines mean-field electrostatic and phenomenological nonelectrostatic interactions, while the number of dissociated counterions is treated as a variable degree of freedom that is determined self-con...

BACKGROUND Diffuse lamellar keratitis (DLK) is a relatively new syndrome that is increasingly being reported after LASIK. We have observed that a hyperopic shift may be associated with the occurrence of this diffuse lamellar keratitis. CASE PRESENTATION A 26 year old man developed bilateral diffuse lamellar keratitis (DLK) following myopic LASIK. The residual refractive error was +0.5D OD and...

BACKGROUND To perform lamellar keratolimbal allograft transplantation in a one-step procedure with a single graft, we investigated the feasibility of harvesting eccentric lamellar keratolimbal grafts from conventionally processed corneoscleral buttons using a manually guided microkeratome in conjunction with an artificial anterior chamber system. METHODS We used the Moria LSK-One microkeratom...

Cryogenic transmission electron microscopy is an important technique for investigation of nano-structures formed by amphiphilic molecules in dilute aqueous solution. The basic principle and applicability of the method as well as sample preparation, interpretation of images and some artefacts are briefly outlined. The introductory section is followed by structural characterization of aqueous dis...

Improved knowledge of the topology of lamellar bodies is a prerequisite for a molecular-level understanding of skin barrier formation, which in turn may provide clues as to the underlying causes of barrier-deficient skin disease. The aim of this study was to examine the key question of continuity vs. discreteness of the lamellar body system using 3 highly specialized and complementary 3-dimensi...

We study morphologies of thin-film diblock copolymers between two flat and parallel walls. The study is restricted to the weak segregation regime below the order-disorder transition temperature. The deviation from perfect lamellar shape is calculated for phases which are perpendicular and parallel to the walls. We examine the undulations of the inter material dividing surface and its angle with...