The basement lamella under the epidermis of amphibian larvae shows a sub-microscopic architecture of remarkable geometric regularity: It consists of about twenty layers of ground substance in which cylindrical fibers (presumably collagenous) of about 500 Angström diameter are embedded parallel to one another, but with the fiber directions alternating by 90 degrees from layer to layer. The repai...

This paper concerns the drainage of a thin liquid lamella into a Plateau border. Many models for draining soap films assume that their gas-liquid interfaces are effectively immobile. Such models predict a phenomenon known as “marginal pinching”, in which the film tends to pinch in the margin between the lamella and the Plateau border. We analyse the opposite extreme, in which the gas-liquid int...

Preparation of Transmission Electron Microscope (TEM) samples by Focused Ion Beam (FIB) milling is one of the most precise techniques now routinely used for example in failure analysis or material science. These TEM samples are commonly prepared using Ga FIB technology, starting more than 20 years ago [13]. Presently FIB columns are commonly combined with the Scanning Electron Microscopy (SEM) ...

Rodent hippocampal slices of < or = 0.5 mm thickness have been widely used as a convenient in vitro model since the 1970s. However, spontaneous population rhythmic activities do not consistently occur in this preparation due to limited network connectivity. To overcome this limitation, we develop a novel slice preparation of 1 mm thickness from adult mouse hippocampus by separating dentate gyru...

PURPOSE The demands for precut lamellar grafts for Descemet stripping automated endothelial keratoplasty rose in our eye bank from 74 in 2007 to 408 in 2010. To meet this expanding requirement, we explored the possibility to preserve these preparations in organ culture. METHODS Organ cultured corneas, stored in a medium containing 6% dextran, were mounted on a Moria artificial anterior chambe...

The need of aquarists for proper and safe disinfection of fish with Chloramine-T (CL-T) necessitates toxico/pathologic examinations.   This study assays toxicity level of CL-T and histopathologic changes of gills, liver, kidney and brain of treated zebrafish (Danio rerio). Groups of 10 Zebrafish were treated with 0 (control), 5, 10, 15, 30, 50,100 and 200 mg/L of CL– T. Mortality was recorded a...

Abstract Mucous cells, the microscopic structural hallmark of mucosal surfaces, are highly responsive to environmental changes. Here we report how gills Atlantic salmon (Salmo salar) smolts responded peracetic acid (PAA), a potent oxidative disinfectant and candidate chemotherapeutant in aquaculture, through Mucosal Mapping showing mucous cell size, volumetric density defence activity, coupled ...